2f30: Difference between revisions

Revision as of 03:24, 4 May 2008

Triclinic cross-linked Lysozyme soaked with 4.5M urea

OverviewOverview

Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (>or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect).

About this StructureAbout this Structure

2F30 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations., Salem M, Mauguen Y, Prange T, Biochim Biophys Acta. 2006 May;1764(5):903-12. Epub 2006 Mar 20. PMID:16600702 Page seeded by OCA on Sun May 4 03:24:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2f30.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2f30 |SIZE=350|CAPTION= <scene name='initialview01'>2f30</scene>, resolution 1.65&Aring;
+The line below this paragraph, containing "STRUCTURE_2f30", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2f30|  PDB=2f30  |  SCENE=  }}
-|RELATEDENTRY=[[2f2n|2F2N]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f30 OCA], [http://www.ebi.ac.uk/pdbsum/2f30 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f30 RCSB]</span>
-}}
 '''Triclinic cross-linked Lysozyme soaked with 4.5M urea'''
@@ Line 28: / Line 25: @@
 [[Category: Prange, T.]]
 [[Category: Salem, M.]]
-[[Category: barnase]]
+[[Category: Barnase]]
-[[Category: bromoethanol]]
+[[Category: Bromoethanol]]
-[[Category: cross-linked crystal]]
+[[Category: Cross-linked crystal]]
-[[Category: denaturation]]
+[[Category: Denaturation]]
-[[Category: glutaraldehyde]]
+[[Category: Glutaraldehyde]]
-[[Category: lysozyme]]
+[[Category: Lysozyme]]
-[[Category: thiourea]]
+[[Category: Thiourea]]
-[[Category: urea]]
+[[Category: Urea]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:24:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:57:11 2008''