2ezr: Difference between revisions

Revision as of 03:17, 4 May 2008

SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 21-30 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES

OverviewOverview

The solution structure of the ectodomain of simian immunodeficiency virus (SIV) gp41 (e-gp41), consisting of residues 27-149, has been determined by multidimensional heteronuclear NMR spectroscopy. SIV e-gp41 is a symmetric 44 kDa trimer with each subunit consisting of antiparallel N-terminal (residues 30-80) and C-terminal (residues 107-147) helices connected by a 26 residue loop (residues 81-106). The N-terminal helices of each subunit form a parallel coiled-coil structure in the interior of the complex which is surrounded by the C-terminal helices located on the exterior of the complex. The loop region is ordered and displays numerous intermolecular and non-sequential intramolecular contacts. The helical core of SIV e-gp41 is similar to recent X-ray structures of truncated constructs of the helical core of HIV-1 e-gp41. The present structure establishes unambiguously the connectivity of the N- and C-terminal helices in the trimer, and characterizes the conformation of the intervening loop, which has been implicated by mutagenesis and antibody epitope mapping to play a key role in gp120 association. In conjunction with previous studies, the solution structure of the SIV e-gp41 ectodomain provides insight into the binding site of gp120 and the mechanism of cell fusion. The present structure of SIV e-gp41 represents one of the largest protein structures determined by NMR to date.

About this StructureAbout this Structure

2EZR is a Single protein structure of sequence from Simian immunodeficiency virus. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41., Caffrey M, Cai M, Kaufman J, Stahl SJ, Wingfield PT, Covell DG, Gronenborn AM, Clore GM, EMBO J. 1998 Aug 17;17(16):4572-84. PMID:9707417 Page seeded by OCA on Sun May 4 03:17:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2ezr.gif|left|200px]]
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ezr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ezr OCA], [http://www.ebi.ac.uk/pdbsum/2ezr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ezr RCSB]</span>
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 '''SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 21-30 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES'''
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 [[Category: Clore, G M.]]
 [[Category: Gronenborn, A M.]]
-[[Category: siv gp41 ectodomain]]
+[[Category: Siv gp41 ectodomain]]
-[[Category: virus envelope protein]]
+[[Category: Virus envelope protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:17:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:55:55 2008''