2ez0: Difference between revisions
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'''Crystal structure of the S107A/E148Q/Y445A mutant of EcClC, in complex with a FaB fragment''' | '''Crystal structure of the S107A/E148Q/Y445A mutant of EcClC, in complex with a FaB fragment''' | ||
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[[Category: Dutzler, R.]] | [[Category: Dutzler, R.]] | ||
[[Category: Lobet, S.]] | [[Category: Lobet, S.]] | ||
[[Category: | [[Category: Clc family of channels and transporter]] | ||
[[Category: | [[Category: H+/cl- antiporter]] | ||
[[Category: | [[Category: Membrane protein/fab complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:16:13 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 03:16, 4 May 2008
Crystal structure of the S107A/E148Q/Y445A mutant of EcClC, in complex with a FaB fragment
OverviewOverview
The ClC channels are members of a large protein family of chloride (Cl-) channels and secondary active Cl- transporters. Despite their diverse functions, the transmembrane architecture within the family is conserved. Here we present a crystallographic study on the ion-binding properties of the ClC selectivity filter in the close homolog from Escherichia coli (EcClC). The ClC selectivity filter contains three ion-binding sites that bridge the extra- and intracellular solutions. The sites bind Cl- ions with mM affinity. Despite their close proximity within the filter, the three sites can be occupied simultaneously. The ion-binding properties are found conserved from the bacterial transporter EcClC to the human Cl- channel ClC-1, suggesting a close functional link between ion permeation in the channels and active transport in the transporters. In resemblance to K+ channels, ions permeate the ClC channel in a single file, with mutual repulsion between the ions fostering rapid conduction.
About this StructureAbout this Structure
2EZ0 is a Single protein structure of sequence from Escherichia coli and Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Ion-binding properties of the ClC chloride selectivity filter., Lobet S, Dutzler R, EMBO J. 2006 Jan 11;25(1):24-33. Epub 2005 Dec 8. PMID:16341087 Page seeded by OCA on Sun May 4 03:16:13 2008