2ex5: Difference between revisions

Revision as of 03:12, 4 May 2008

Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA

OverviewOverview

Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.

About this StructureAbout this Structure

2EX5 is a Single protein structure of sequence from Chlamydomonas eugametos. Full crystallographic information is available from OCA.

ReferenceReference

The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold., Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL, Structure. 2006 May;14(5):869-80. PMID:16698548 Page seeded by OCA on Sun May 4 03:12:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2ex5.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2ex5 |SIZE=350|CAPTION= <scene name='initialview01'>2ex5</scene>, resolution 2.200&Aring;
+The line below this paragraph, containing "STRUCTURE_2ex5", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= I-CeuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3053 Chlamydomonas eugametos])
+-->
-|DOMAIN=
+{{STRUCTURE_2ex5|  PDB=2ex5  |  SCENE=  }}
-|RELATEDENTRY=[[1af5|1AF5]], [[1bp7|1BP7]], [[1g9y|1G9Y]], [[1g9z|1G9Z]], [[1m5x|1M5X]], [[1mow|1MOW]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex5 OCA], [http://www.ebi.ac.uk/pdbsum/2ex5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ex5 RCSB]</span>
-}}
 '''Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA'''
@@ Line 27: / Line 24: @@
 [[Category: Spiegel, P C.]]
 [[Category: Stoddard, B L.]]
-[[Category: homing endonuclease]]
+[[Category: Homing endonuclease]]
-[[Category: homodimer]]
+[[Category: Homodimer]]
-[[Category: laglidadg]]
+[[Category: Laglidadg]]
-[[Category: protein/dna complex]]
+[[Category: Protein/dna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:12:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:54:57 2008''