2erm: Difference between revisions

Revision as of 03:02, 4 May 2008

Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue

OverviewOverview

The 3D structure of a complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed synthetic heparin hexasaccharide has been determined by NMR spectroscopy. This hexasaccharide can substitute natural heparins in FGF-1 mitogenesis assays, in spite of not inducing any apparent dimerization of the growth factor. The use of this well defined synthetic heparin analogue has allowed us to perform a detailed NMR structural analysis of the heparin-FGF interaction, overcoming the limitations of NMR to deal with the high molecular mass and heterogeneity of the FGF-1 oligomers formed in the presence of natural heparin fragments. Our results confirm that glycosaminoglycans induced FGF-1 dimerization either in a cis or trans disposition with respect to the heparin chain is not an absolute requirement for biological activity.

About this StructureAbout this Structure

2ERM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue., Canales A, Lozano R, Lopez-Mendez B, Angulo J, Ojeda R, Nieto PM, Martin-Lomas M, Gimenez-Gallego G, Jimenez-Barbero J, FEBS J. 2006 Oct;273(20):4716-27. Epub 2006 Sep 21. PMID:16995857 Page seeded by OCA on Sun May 4 03:02:19 2008

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OCA

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 '''Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue'''
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 [[Category: Martin-Lomas, M.]]
 [[Category: Nieto, P M.]]
-[[Category: fibroblast growth factor]]
+[[Category: Fibroblast growth factor]]
-[[Category: heparin-like hexasaccharide]]
+[[Category: Heparin-like hexasaccharide]]
-[[Category: protein-carbohydrate complex]]
+[[Category: Protein-carbohydrate complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:02:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:52:54 2008''