2enr: Difference between revisions

Revision as of 02:51, 4 May 2008

CO-CRYSTALS OF DEMETALLIZED CONCANAVALIN A WITH CADMIUM HAVING A CADMIUM ION BOUND IN BOTH THE S1 SITE AND THE S2 SITE

OverviewOverview

The crystal structures of cadmium/cadmium and zinc/calcium concanavalin A (con A) at pH 5.0 and pH 6.15, respectively, were determined. The structure of cadmium/cadmium con A confirms that the secondary Cd(2+)-binding site S3 is empty at pH 5. The metal-binding sites S1 and S2 are only very slightly affected by the substitution with cadmium. On the other hand, S1 and S2 and most of the protein surface of zinc/calcium con A at pH 6.15 differ from other fully metal-bound and carbohydrate-free structures. Most of these structural differences at the protein surface are a result of the interplay between metal binding, protonation and crystal packing. This interplay is expressed by relative rotations and translations of the con A units in alternative crystal packings and participation in space-group conversions inside crystals in situ. The particular crystal packing of zinc/calcium con A creates a novel zinc-binding site S4. The Zn(2+) ion in S4 ligates two aspartates from one tetramer and a histidine from a symmetry-related tetramer.

About this StructureAbout this Structure

2ENR is a Single protein structure of sequence from Canavalia ensiformis. Full crystallographic information is available from OCA.

ReferenceReference

Zinc/calcium- and cadmium/cadmium-substituted concanavalin A: interplay of metal binding, pH and molecular packing., Bouckaert J, Loris R, Wyns L, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1569-76. PMID:11092923 Page seeded by OCA on Sun May 4 02:51:17 2008

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OCA

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 [[Image:2enr.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2enr |SIZE=350|CAPTION= <scene name='initialview01'>2enr</scene>, resolution 2.35&Aring;
+The line below this paragraph, containing "STRUCTURE_2enr", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=S1:Transition+Metal+Binding+Site+S1+6-Coordinated+Octahedra+...'>S1</scene> and <scene name='pdbsite=S2:Ca+Binding+Site+S2+7-Coordinated+Distorted+Octahedral+Co+...'>S2</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2enr|  PDB=2enr  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2enr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2enr OCA], [http://www.ebi.ac.uk/pdbsum/2enr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2enr RCSB]</span>
-}}
 '''CO-CRYSTALS OF DEMETALLIZED CONCANAVALIN A WITH CADMIUM HAVING A CADMIUM ION BOUND IN BOTH THE S1 SITE AND THE S2 SITE'''
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 [[Category: Loris, R.]]
 [[Category: Wyns, L.]]
-[[Category: cadmium]]
+[[Category: Cadmium]]
-[[Category: carbohydrate binding]]
+[[Category: Carbohydrate binding]]
-[[Category: concanavalin some]]
+[[Category: Concanavalin some]]
-[[Category: metal binding]]
+[[Category: Metal binding]]
-[[Category: plant lectin]]
+[[Category: Plant lectin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 02:51:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:51:32 2008''