2e2i: Difference between revisions

Revision as of 01:48, 4 May 2008

RNA polymerase II elongation complex in 5 mM Mg+2 with 2'-dGTP

OverviewOverview

New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.

About this StructureAbout this Structure

2E2I is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis., Wang D, Bushnell DA, Westover KD, Kaplan CD, Kornberg RD, Cell. 2006 Dec 1;127(5):941-54. PMID:17129781 Page seeded by OCA on Sun May 4 01:48:00 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2e2i.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2e2i |SIZE=350|CAPTION= <scene name='initialview01'>2e2i</scene>, resolution 3.41&Aring;
+The line below this paragraph, containing "STRUCTURE_2e2i", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DGT:2&#39;-DEOXYGUANOSINE-5&#39;-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2e2i|  PDB=2e2i  |  SCENE=  }}
-|RELATEDENTRY=[[1sfo|1SFO]], [[1r9s|1R9S]], [[1r9t|1R9T]], [[2e2h|2E2H]], [[2e2j|2E2J]], [[2nvq|2NVQ]], [[2nvs|2NVS]], [[2nvt|2NVT]], [[2nvx|2NVX]], [[2nvy|2NVY]], [[2nvz|2NVZ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e2i OCA], [http://www.ebi.ac.uk/pdbsum/2e2i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2e2i RCSB]</span>
-}}
 '''RNA polymerase II elongation complex in 5 mM Mg+2 with 2'-dGTP'''
@@ Line 31: / Line 28: @@
 [[Category: Wang, D.]]
 [[Category: Westover, K D.]]
-[[Category: dna]]
+[[Category: Dna]]
-[[Category: molecular machine]]
+[[Category: Molecular machine]]
-[[Category: mrna]]
+[[Category: Mrna]]
-[[Category: multiprotein complex]]
+[[Category: Multiprotein complex]]
-[[Category: transcription]]
+[[Category: Transcription]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:48:00 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:42:45 2008''