2dt9: Difference between revisions

Revision as of 01:18, 4 May 2008

Crystal structure of the regulatory subunit of aspartate kinase from Thermus flavus

OverviewOverview

To reveal the catalytic mechanism of Thermus aspartate kinase, each of 29 amino acid residues that were highly conserved in the sequenced aspartate kinases, was replaced with alanine or leucine by PCR site-directed mutagenesis. Comparison of the kinetic parameters of these mutants with those of the wild-type aspartate kinase suggested that Thr47 was involved in binding aspartate and that Lys7 and Glu74 were involved in catalysis. Analysis of the effective concentrations of magnesium ion on the activity showed that the mutants with replacements at Ser41, Thr47, Asp154 and Asp182 required higher concentrations of magnesium ion. This suggests that these four residues play important roles in the binding of magnesium ions which are required for enzymatic activity.

About this StructureAbout this Structure

2DT9 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Kinetic and mutation analyses of aspartate kinase from Thermus flavus., Kobashi N, Nishiyama M, Tanokura M, J Biosci Bioeng. 1999;87(6):739-45. PMID:16232547 Page seeded by OCA on Sun May 4 01:18:29 2008

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OCA

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 [[Image:2dt9.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2dt9 |SIZE=350|CAPTION= <scene name='initialview01'>2dt9</scene>, resolution 2.15&Aring;
+The line below this paragraph, containing "STRUCTURE_2dt9", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= askB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
+-->
-|DOMAIN=
+{{STRUCTURE_2dt9|  PDB=2dt9  |  SCENE=  }}
-|RELATEDENTRY=[[2dtj|2DTJ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dt9 OCA], [http://www.ebi.ac.uk/pdbsum/2dt9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dt9 RCSB]</span>
-}}
 '''Crystal structure of the regulatory subunit of aspartate kinase from Thermus flavus'''
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 [[Category: Tomita, T.]]
 [[Category: Yoshida, A.]]
-[[Category: protein-ligand complex]]
+[[Category: Protein-ligand complex]]
-[[Category: regulatory subunit]]
+[[Category: Regulatory subunit]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:18:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:39:10 2008''