2ds6: Difference between revisions

Revision as of 01:05, 4 May 2008

Structure of the ZBD in the tetragonal crystal form

OverviewOverview

The degradation of ssrA(AANDENYALAA)-tagged proteins in the bacterial cytosol is carried out by the ClpXP protease and is markedly stimulated by the SspB adaptor protein. It has previously been reported that the amino-terminal zinc-binding domain of ClpX (ZBD) is involved in complex formation with the SspB-tail (XB: ClpX-binding motif). In an effort to better understand the recognition of SspB by ClpX and the mechanism of delivery of ssrA-tagged substrates to ClpXP, we have determined the structures of ZBD alone at 1.5, 2.0, and 2.5 A resolution in each different crystal form and also in complex with XB peptide at 1.6 A resolution. The XB peptide forms an antiparallel beta-sheet with two beta-strands of ZBD, and the structure shows a 1:1 stoichiometric complex between ZBD and XB, suggesting that there are two independent SspB-tail-binding sites in ZBD. The high-resolution ZBD:XB complex structure, in combination with biochemical analyses, can account for key determinants in the recognition of the SspB-tail by ClpX and sheds light on the mechanism of delivery of target proteins to the prokaryotic degradation machine.

About this StructureAbout this Structure

2DS6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of SspB-tail recognition by the zinc binding domain of ClpX., Park EY, Lee BG, Hong SB, Kim HW, Jeon H, Song HK, J Mol Biol. 2007 Mar 23;367(2):514-26. Epub 2007 Jan 9. PMID:17258768 Page seeded by OCA on Sun May 4 01:05:47 2008

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OCA

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 [[Image:2ds6.gif|left|200px]]
- {{Structure
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-}}
 '''Structure of the ZBD in the tetragonal crystal form'''
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 [[Category: Park, E Y.]]
 [[Category: Song, H K.]]
-[[Category: substrate binding domain]]
+[[Category: Substrate binding domain]]
-[[Category: zinc finger domain]]
+[[Category: Zinc finger domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:05:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:43 2008''