2ds0: Difference between revisions

Revision as of 01:04, 4 May 2008

Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose

OverviewOverview

Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.

About this StructureAbout this Structure

2DS0 is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.

ReferenceReference

Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683 Page seeded by OCA on Sun May 4 01:04:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2ds0.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2ds0 |SIZE=350|CAPTION= <scene name='initialview01'>2ds0</scene>, resolution 1.80&Aring;
+The line below this paragraph, containing "STRUCTURE_2ds0", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Sia+Binding+Site+For+Residue+B+270'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+271'>AC2</scene>, <scene name='pdbsite=AC3:Sia+Binding+Site+For+Residue+A+280'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+281'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+1001'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+1002'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+A+1003'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+A+1004'>AC8</scene> and <scene name='pdbsite=AC9:So4+Binding+Site+For+Residue+B+1005'>AC9</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2ds0|  PDB=2ds0  |  SCENE=  }}
-|RELATEDENTRY=[[2ao3|2AO3]], [[2d12|2D12]], [[2dry|2DRY]], [[2drz|2DRZ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ds0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ds0 OCA], [http://www.ebi.ac.uk/pdbsum/2ds0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ds0 RCSB]</span>
-}}
 '''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose'''
@@ Line 27: / Line 24: @@
 [[Category: Fujimoto, Z.]]
 [[Category: Suzuki, R.]]
-[[Category: beta-trefoil fold]]
+[[Category: Beta-trefoil fold]]
-[[Category: earthworm lumbricus terrestri]]
+[[Category: Earthworm lumbricus terrestri]]
-[[Category: galactose]]
+[[Category: Galactose]]
-[[Category: in vitro evolution]]
+[[Category: In vitro evolution]]
-[[Category: sialic acid]]
+[[Category: Sialic acid]]
-[[Category: sugar binding protein]]
+[[Category: Sugar binding protein]]
-[[Category: sugar complex]]
+[[Category: Sugar complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:04:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:40 2008''