2dpg: Difference between revisions

Revision as of 00:54, 4 May 2008

COMPLEX OF INACTIVE MUTANT (H240->N) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES WITH NADP+

OverviewOverview

The catalytic mechanism of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides was investigated by replacing three amino acids, His-240, Asp-177, and His 178, with asparagine, using site-directed mutagenesis. Each of the mutant enzymes was purified to homogeneity and characterized by substrate binding studies and steady-state kinetic analyses. The three-dimensional structure of the H240N glucose 6-phosphate dehydrogenase was determined at 2.5 A resolution. The results support a mechanism in which His-240 acts as the general base that abstracts the proton from the C1-hydroxyl group of glucose 6-phosphate, and the carboxylate group of Asp-177 stabilizes the positive charge that forms on His-240 in the transition state. The results also confirm the postulated role of His-178 in binding the phosphate moiety of glucose 6-phosphate.

About this StructureAbout this Structure

2DPG is a Single protein structure of sequence from Leuconostoc mesenteroides. Full crystallographic information is available from OCA.

ReferenceReference

On the mechanism of the reaction catalyzed by glucose 6-phosphate dehydrogenase., Cosgrove MS, Naylor C, Paludan S, Adams MJ, Levy HR, Biochemistry. 1998 Mar 3;37(9):2759-67. PMID:9485426 Page seeded by OCA on Sun May 4 00:54:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2dpg.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2dpg |SIZE=350|CAPTION= <scene name='initialview01'>2dpg</scene>, resolution 2.5&Aring;
+The line below this paragraph, containing "STRUCTURE_2dpg", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=NUL:Active+Site+Mutant,+HIS+240+-&#62;+ASN.+The+Base+Required+To+...'>NUL</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_1-dehydrogenase Glucose-6-phosphate 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.49 1.1.1.49] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PLMZ/H240N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1245 Leuconostoc mesenteroides])
+-->
-|DOMAIN=
+{{STRUCTURE_2dpg|  PDB=2dpg  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dpg OCA], [http://www.ebi.ac.uk/pdbsum/2dpg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dpg RCSB]</span>
-}}
 '''COMPLEX OF INACTIVE MUTANT (H240->N) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES WITH NADP+'''
@@ Line 30: / Line 27: @@
 [[Category: Naylor, C E.]]
 [[Category: Paludin, S.]]
-[[Category: choh(d) - nad(p)]]
+[[Category: Glucose metabolism]]
-[[Category: glucose metabolism]]
+[[Category: Nadp/nad]]
-[[Category: nadp/nad]]
+[[Category: Oxidoreductase]]
-[[Category: oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:54:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:37:39 2008''