2dea: Difference between revisions

Revision as of 00:15, 4 May 2008

Crystal Structure of the Aminopeptidase of Aeromonas proteolytica at pH 4.7

OverviewOverview

The aminopeptidase from Aeromonas proteolytica (AAP) contains two zinc ions in the active site and catalyzes the degradation of peptides. Herein we report the crystal structures of AAP at 0.95-A resolution at neutral pH and at 1.24-A resolution at low pH. The combination of these structures allowed the precise modeling of atomic positions, the identification of the metal bridging oxygen species, and insight into the physical properties of the metal ions. On the basis of these structures, a new putative catalytic mechanism is proposed for AAP that is likely relevant to all binuclear metalloproteases.

About this StructureAbout this Structure

2DEA is a Single protein structure of sequence from Vibrio proteolyticus. Full crystallographic information is available from OCA.

ReferenceReference

The high-resolution structures of the neutral and the low pH crystals of aminopeptidase from Aeromonas proteolytica., Desmarais W, Bienvenue DL, Bzymek KP, Petsko GA, Ringe D, Holz RC, J Biol Inorg Chem. 2006 Jun;11(4):398-408. Epub 2006 Apr 5. PMID:16596389 Page seeded by OCA on Sun May 4 00:15:55 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2dea.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2dea |SIZE=350|CAPTION= <scene name='initialview01'>2dea</scene>, resolution 1.24&Aring;
+The line below this paragraph, containing "STRUCTURE_2dea", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bacterial_leucyl_aminopeptidase Bacterial leucyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.10 3.4.11.10] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2dea|  PDB=2dea  |  SCENE=  }}
-|RELATEDENTRY=[[1lok|1LOK]], [[1rtq|1RTQ]], [[1ft7|1FT7]], [[1txr|1TXR]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dea OCA], [http://www.ebi.ac.uk/pdbsum/2dea PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dea RCSB]</span>
-}}
 '''Crystal Structure of the Aminopeptidase of Aeromonas proteolytica at pH 4.7'''
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 [[Category: Petsko, G A.]]
 [[Category: Ringe, D.]]
-[[Category: aminopeptidase]]
+[[Category: Aminopeptidase]]
-[[Category: bimetalloenzyme]]
+[[Category: Bimetalloenzyme]]
-[[Category: low ph]]
+[[Category: Low ph]]
-[[Category: metalloenzyme]]
+[[Category: Metalloenzyme]]
-[[Category: zinc enzyme]]
+[[Category: Zinc enzyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:15:55 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:33:35 2008''