2d3v: Difference between revisions

Revision as of 23:39, 3 May 2008

Crystal Structure of Leukocyte Ig-like Receptor A5 (LILRA5/LIR9/ILT11)

OverviewOverview

Human leukocyte Ig-like receptor B1 (LILRB1) and B2 (LILRB2) belong to "Group 1" receptors and recognize a broad range of major histocompatibility complex class I molecules (MHCIs). In contrast, "Group 2" receptors show low similarity with LILRB1/B2, and their ligands remain to be identified. To date, the structural and functional characteristics of Group 2 LILRs are poorly understood. Here we report the crystal structure of the extracellular domain of LILRA5, which is an activating Group 2 LILR expressed on monocytes and neutrophils. Unexpectedly, the structure showed large changes in structural conformation and charge distribution in the region corresponding to the MHCI binding site of LILRB1/B2, which are also distinct from killer cell Ig-like receptors and Fc alpha receptors. These changes probably confer the structural hindrance for the MHCI binding, and their key amino acid substitutions are well conserved in Group 2 LILRs. Consistently, the surface plasmon resonance and flow cytometric analyses demonstrated that LILRA5 exhibited no affinities to all tested MHCIs. These results raised the possibility that LILRA5 as well as Group 2 LILRs do not play a role in any MHCI recognition but could possibly bind to non-MHCI ligand(s) on the target cells to provide a novel immune regulation mechanism.

About this StructureAbout this Structure

2D3V is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the human monocyte-activating receptor, "Group 2" leukocyte Ig-like receptor A5 (LILRA5/LIR9/ILT11)., Shiroishi M, Kajikawa M, Kuroki K, Ose T, Kohda D, Maenaka K, J Biol Chem. 2006 Jul 14;281(28):19536-44. Epub 2006 May 3. PMID:16675463 Page seeded by OCA on Sat May 3 23:39:25 2008

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OCA

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 '''Crystal Structure of Leukocyte Ig-like Receptor A5 (LILRA5/LIR9/ILT11)'''
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 [[Category: Ose, T.]]
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-[[Category: immunoglobulin-like fold]]
+[[Category: Immunoglobulin-like fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:39:25 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:29:58 2008''