2d3t: Difference between revisions

Revision as of 23:39, 3 May 2008

Fatty Acid beta-oxidation multienzyme complex from Pseudomonas Fragi, Form V

OverviewOverview

The quaternary structure of a fatty acid beta-oxidation multienzyme complex, catalyzing three sequential reactions, was investigated by X-ray crystallographic and small-angle X-ray solution scattering analyses. X-ray crystallography revealed an intermediate structure of the complex among the previously reported structures. However, the theoretical scattering curves calculated from the crystal structures remarkably disagree with the experimental profiles. Instead, an ensemble of the atomic models, which were all calculated by rigid-body optimization, reasonably explained the experimental data. These structures significantly differ from those in the crystals, but they maintain the substrate binding pocket at the domain boundary. Comparisons among these structures indicated that binding of 3-hydroxyhexadecanoyl-CoA or nicotinamide adenine dinucleotide induces domain rearrangements in the complex. The conformational changes suggest the structural events occurring during the chain reaction catalyzed by the multienzyme complex.

About this StructureAbout this Structure

2D3T is a Protein complex structure of sequences from Pseudomonas fragi. Full crystallographic information is available from OCA.

ReferenceReference

Ligand-induced domain rearrangement of fatty acid beta-oxidation multienzyme complex., Tsuchiya D, Shimizu N, Ishikawa M, Suzuki Y, Morikawa K, Structure. 2006 Feb;14(2):237-46. PMID:16472743 Page seeded by OCA on Sat May 3 23:39:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2d3t.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2d3t |SIZE=350|CAPTION= <scene name='initialview01'>2d3t</scene>, resolution 3.4&Aring;
+The line below this paragraph, containing "STRUCTURE_2d3t", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetyl-CoA_C-acyltransferase Acetyl-CoA C-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.16 2.3.1.16] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2d3t|  PDB=2d3t  |  SCENE=  }}
-|RELATEDENTRY=[[1wdk|1WDK]], [[1wdl|1WDL]], [[1wdm|1WDM]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3t OCA], [http://www.ebi.ac.uk/pdbsum/2d3t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2d3t RCSB]</span>
-}}
 '''Fatty Acid beta-oxidation multienzyme complex from Pseudomonas Fragi, Form V'''
@@ Line 31: / Line 28: @@
 [[Category: Suzuki, Y.]]
 [[Category: Tsuchiya, D.]]
-[[Category: alpha2beta2 heterotetrameric complex]]
+[[Category: Alpha2beta2 heterotetrameric complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:39:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:29:56 2008''