2d3o: Difference between revisions

Revision as of 23:38, 3 May 2008

Structure of Ribosome Binding Domain of the Trigger Factor on the 50S ribosomal subunit from D. radiodurans

OverviewOverview

This study presents the X-ray structure of the N-terminal binding domain of the D. radiodurans trigger factor (TF) in complex with the D. radiodurans large ribosomal subunit. At 3.35 A, a complete description of the interactions with ribosomal proteins L23, L29, and 23S rRNA are disclosed, many of which differ from those found previously for a heterologous bacterial-archaeal TF-ribosome complex. The beta hairpin loop of eubacterial L24, which is shorter in archaeal ribosomes, contacts the TF and severely diminishes the molecular cradle proposed to exist between the TF and ribosome. Bound to the ribosome, TF exposes a hydrophobic crevice large enough to accommodate the nascent polypeptide chain. Superimposition of the full-length TF and the signal-recognition particle (SRP) onto the complex shows that simultaneous cohabitation is possible, in agreement with biochemical data, and suggests a model for the interplay of TF, SRP, and the nascent chain during translation.

About this StructureAbout this Structure

2D3O is a Protein complex structure of sequences from Deinococcus radiodurans. Full crystallographic information is available from OCA.

ReferenceReference

The binding mode of the trigger factor on the ribosome: implications for protein folding and SRP interaction., Schlunzen F, Wilson DN, Tian P, Harms JM, McInnes SJ, Hansen HA, Albrecht R, Buerger J, Wilbanks SM, Fucini P, Structure. 2005 Nov;13(11):1685-94. PMID:16271892 Page seeded by OCA on Sat May 3 23:38:37 2008

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OCA

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 [[Image:2d3o.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2d3o", creates the "Structure Box" on the page.
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3o OCA], [http://www.ebi.ac.uk/pdbsum/2d3o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2d3o RCSB]</span>
-}}
 '''Structure of Ribosome Binding Domain of the Trigger Factor on the 50S ribosomal subunit from D. radiodurans'''
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 [[Category: Wilson, D N.]]
 [[Category: 50]]
-[[Category: nascent chain]]
+[[Category: Nascent chain]]
-[[Category: protein folding]]
+[[Category: Protein folding]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
-[[Category: srp]]
+[[Category: Srp]]
-[[Category: trigger factor]]
+[[Category: Trigger factor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:38:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:29:52 2008''