2cv5: Difference between revisions

Revision as of 23:08, 3 May 2008

Crystal structure of human nucleosome core particle

OverviewOverview

Gene expression in eukaryotes depends upon positioning, mobility and packaging of nucleosomes; thus, we need the detailed information of the human nucleosome core particle (NCP) structure, which could clarify chromatin properties. Here, we report the 2.5 A crystal structure of a human NCP. The overall structure is similar to those of other NCPs reported previously. However, the DNA path of human NCP is remarkably different from that taken within other NCPs with an identical DNA sequence. A comparison of the structural parameters between human and Xenopus laevis DNA reveals that the DNA path of human NCP consecutively shifts by 1 bp in the regions of superhelix axis location -5.0 to -2.0 and 5.0 to 7.0. This alteration of the human DNA path is caused predominantly by tight DNA-DNA contacts within the crystal. It is also likely that the conformational change in the human H2B tail induces the local alteration of the DNA path. In human NCP, the region with the altered DNA path lacks Mn2+ ions and the B-factors of the DNA phosphate groups are substantially high. Therefore, in contrast to the histone octamer, the nucleosomal DNA is sufficiently flexible and mobile and can undergo drastic conformational changes, depending upon the environment.

About this StructureAbout this Structure

2CV5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Alteration of the nucleosomal DNA path in the crystal structure of a human nucleosome core particle., Tsunaka Y, Kajimura N, Tate S, Morikawa K, Nucleic Acids Res. 2005 Jun 10;33(10):3424-34. Print 2005. PMID:15951514 Page seeded by OCA on Sat May 3 23:08:10 2008

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OCA

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 '''Crystal structure of human nucleosome core particle'''
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 [[Category: Tate, S.]]
 [[Category: Tsunaka, Y.]]
-[[Category: human nucleosome structure]]
+[[Category: Human nucleosome structure]]
-[[Category: metal binding site]]
+[[Category: Metal binding site]]
-[[Category: supercoiled dna path]]
+[[Category: Supercoiled dna path]]
-[[Category: x-ray crystallography]]
+[[Category: X-ray crystallography]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:08:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:26:48 2008''