2clu: Difference between revisions

Revision as of 22:27, 3 May 2008

RECOMBINANT HUMAN H FERRITIN, K86Q AND E107D MUTANT

OverviewOverview

Ferritins are a family of proteins distributed widely in nature. In bacterial, plant, and animal cells, ferritin appears to serve as a soluble, bioavailable, and non-toxic form of iron provider. Ferritins from animal sources are heteropolymers composed of two types of subunit, H and L, which differ mainly by the presence (H) or absence (L) of active ferroxidase centres. We report the crystallographic structures of four human H apoferritin variants at a resolution of up to 1.5 Angstrom. Crystal derivatives using Zn(II) as redox-stable alternative for Fe(II), allows us to characterize the different metal-binding sites. The ferroxidase centre, which is composed of sites A and B, binds metal with a preference for the A site. In addition, distinct Zn(II)-binding sites were found in the 3-fold axes, 4-fold axes and on the cavity surface near the ferroxidase centre. To study the importance of the distance of the two metal atoms in the ferroxidase centre, single and double replacement of glutamate 27 (site A) and glutamate 107 (site B), the two axial ligands, by aspartate residues have been carried out. The consequences for metal binding and the correlation with Fe(II) oxidation rates are discussed.

About this StructureAbout this Structure

2CLU is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

High-resolution X-ray structures of human apoferritin H-chain mutants correlated with their activity and metal-binding sites., Toussaint L, Bertrand L, Hue L, Crichton RR, Declercq JP, J Mol Biol. 2007 Jan 12;365(2):440-52. Epub 2006 Oct 7. PMID:17070541 Page seeded by OCA on Sat May 3 22:27:17 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2clu.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2clu |SIZE=350|CAPTION= <scene name='initialview01'>2clu</scene>, resolution 2.10&Aring;
+The line below this paragraph, containing "STRUCTURE_2clu", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2clu|  PDB=2clu  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2clu OCA], [http://www.ebi.ac.uk/pdbsum/2clu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2clu RCSB]</span>
-}}
 '''RECOMBINANT HUMAN H FERRITIN, K86Q AND E107D MUTANT'''
@@ Line 29: / Line 26: @@
 [[Category: Declercq, J P.]]
 [[Category: Toussaint, L.]]
-[[Category: apoferritin]]
+[[Category: Apoferritin]]
-[[Category: di-iron non-heme protein]]
+[[Category: Di-iron non-heme protein]]
-[[Category: ferroxidase]]
+[[Category: Ferroxidase]]
-[[Category: iron storage]]
+[[Category: Iron storage]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 22:27:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:23:17 2008''