2cea: Difference between revisions

Revision as of 21:56, 3 May 2008

WILDTYPE

OverviewOverview

The ATP-dependent integral membrane protease FtsH is universally conserved in bacteria. Orthologs exist in chloroplasts and mitochondria, where in humans the loss of a close FtsH-homolog causes a form of spastic paraplegia. FtsH plays a crucial role in quality control by degrading unneeded or damaged membrane proteins, but it also targets soluble signaling factors like sigma(32) and lambda-CII. We report here the crystal structure of a soluble FtsH construct that is functional in caseinolytic and ATPase assays. The molecular architecture of this hexameric molecule consists of two rings where the protease domains possess an all-helical fold and form a flat hexagon that is covered by a toroid built by the AAA domains. The active site of the protease classifies FtsH as an Asp-zincin, contrary to a previous report. The different symmetries of protease and AAA rings suggest a possible translocation mechanism of the target polypeptide chain into the interior of the molecule where the proteolytic sites are located.

About this StructureAbout this Structure

2CEA is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

ReferenceReference

The molecular architecture of the metalloprotease FtsH., Bieniossek C, Schalch T, Bumann M, Meister M, Meier R, Baumann U, Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3066-71. Epub 2006 Feb 16. PMID:16484367 Page seeded by OCA on Sat May 3 21:56:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2cea.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2cea |SIZE=350|CAPTION= <scene name='initialview01'>2cea</scene>, resolution 2.75&Aring;
+The line below this paragraph, containing "STRUCTURE_2cea", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+E'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2cea|  PDB=2cea  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cea OCA], [http://www.ebi.ac.uk/pdbsum/2cea PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cea RCSB]</span>
-}}
 '''WILDTYPE'''
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 [[Category: Baumann, U.]]
 [[Category: Bieniossek, C.]]
-[[Category: cell division]]
+[[Category: Cell division]]
-[[Category: cell division protein]]
+[[Category: Cell division protein]]
-[[Category: ftsh]]
+[[Category: Ftsh]]
-[[Category: metalloprotease]]
+[[Category: Metalloprotease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:56:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:20:18 2008''