2caz: Difference between revisions

Revision as of 21:36, 3 May 2008

ESCRT-I CORE

OverviewOverview

ESCRT complexes form the main machinery driving protein sorting from endosomes to lysosomes. Currently, the picture regarding assembly of ESCRTs on endosomes is incomplete. The structure of the conserved heterotrimeric ESCRT-I core presented here shows a fan-like arrangement of three helical hairpins, each corresponding to a different subunit. Vps23/Tsg101 is the central hairpin sandwiched between the other subunits, explaining the critical role of its "steadiness box" in the stability of ESCRT-I. We show that yeast ESCRT-I links directly to ESCRT-II, through a tight interaction of Vps28 (ESCRT-I) with the yeast-specific zinc-finger insertion within the GLUE domain of Vps36 (ESCRT-II). The crystal structure of the GLUE domain missing this insertion reveals it is a split PH domain, with a noncanonical lipid binding pocket that binds PtdIns3P. The simultaneous and reinforcing interactions of ESCRT-II GLUE domain with membranes, ESCRT-I, and ubiquitin are critical for ubiquitinated cargo progression from early to late endosomes.

About this StructureAbout this Structure

2CAZ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes., Teo H, Gill DJ, Sun J, Perisic O, Veprintsev DB, Vallis Y, Emr SD, Williams RL, Cell. 2006 Apr 7;125(1):99-111. PMID:16615893 Page seeded by OCA on Sat May 3 21:36:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2caz.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2caz", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE=
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-|DOMAIN=
+{{STRUCTURE_2caz|  PDB=2caz  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2caz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2caz OCA], [http://www.ebi.ac.uk/pdbsum/2caz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2caz RCSB]</span>
-}}
 '''ESCRT-I CORE'''
@@ Line 33: / Line 30: @@
 [[Category: Veprintsev, D B.]]
 [[Category: Williams, R L.]]
-[[Category: coiled coil]]
+[[Category: Coiled coil]]
-[[Category: endosome]]
+[[Category: Endosome]]
-[[Category: escrt]]
+[[Category: Escrt]]
-[[Category: lysosome]]
+[[Category: Lysosome]]
-[[Category: multivesicular body]]
+[[Category: Multivesicular body]]
-[[Category: mvb]]
+[[Category: Mvb]]
-[[Category: ph domain]]
+[[Category: Ph domain]]
-[[Category: phosphoinositide]]
+[[Category: Phosphoinositide]]
-[[Category: protein sorting]]
+[[Category: Protein sorting]]
-[[Category: protein transport]]
+[[Category: Protein transport]]
-[[Category: ptdins3p]]
+[[Category: Ptdins3p]]
-[[Category: ubiquitin]]
+[[Category: Ubiquitin]]
-[[Category: ubl conjugation pathway]]
+[[Category: Ubl conjugation pathway]]
-[[Category: vesicle trafficking]]
+[[Category: Vesicle trafficking]]
-[[Category: vps23]]
+[[Category: Vps23]]
-[[Category: vps28]]
+[[Category: Vps28]]
-[[Category: vps36]]
+[[Category: Vps36]]
-[[Category: vps37]]
+[[Category: Vps37]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:36:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:18:57 2008''