2c7n: Difference between revisions

Revision as of 21:23, 3 May 2008

HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN

OverviewOverview

The interaction between ubiquitinated proteins and intracellular proteins harboring ubiquitin binding domains (UBDs) is critical to a multitude of cellular processes. Here, we report that Rabex-5, a guanine nucleotide exchange factor for Rab5, binds to Ub through two independent UBDs. These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated EGFRs. Structural and biochemical characterization of the UBDs of Rabex-5 revealed that one of them (MIU, motif interacting with ubiquitin) binds to Ub with modes superimposable to those of the UIM (ubiquitin-interacting motif):Ub interaction, although in the opposite orientation. The other UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of Ub centered on Asp58(Ub) and distinct from the "canonical" Ile44(Ub)-based surface. The two binding surfaces allow Ub to interact simultaneously with different UBDs, thus opening new perspectives in Ub-mediated signaling.

About this StructureAbout this Structure

2C7N is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin., Penengo L, Mapelli M, Murachelli AG, Confalonieri S, Magri L, Musacchio A, Di Fiore PP, Polo S, Schneider TR, Cell. 2006 Mar 24;124(6):1183-95. Epub 2006 Feb 23. PMID:16499958 Page seeded by OCA on Sat May 3 21:23:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2c7n.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2c7n |SIZE=350|CAPTION= <scene name='initialview01'>2c7n</scene>, resolution 2.10&Aring;
+The line below this paragraph, containing "STRUCTURE_2c7n", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+K'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2c7n|  PDB=2c7n  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c7n OCA], [http://www.ebi.ac.uk/pdbsum/2c7n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c7n RCSB]</span>
-}}
 '''HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN'''
@@ Line 34: / Line 31: @@
 [[Category: Polo, S.]]
 [[Category: Schneider, T R.]]
-[[Category: endocytosis]]
+[[Category: Endocytosis]]
-[[Category: nuclear protein]]
+[[Category: Nuclear protein]]
-[[Category: polyprotein]]
+[[Category: Polyprotein]]
-[[Category: ubiquitin binding domain]]
+[[Category: Ubiquitin binding domain]]
-[[Category: ubiquitin complex]]
+[[Category: Ubiquitin complex]]
-[[Category: ubl conjugation]]
+[[Category: Ubl conjugation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:23:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:17:31 2008''