2c6z: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2c6z.gif|left|200px]] | [[Image:2c6z.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2c6z", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
| | --> | ||
| | {{STRUCTURE_2c6z| PDB=2c6z | SCENE= }} | ||
}} | |||
'''CRYSTAL STRUCTURE OF DIMETHYLARGININE DIMETHYLAMINOHYDROLASE I IN COMPLEX WITH CITRULLINE''' | '''CRYSTAL STRUCTURE OF DIMETHYLARGININE DIMETHYLAMINOHYDROLASE I IN COMPLEX WITH CITRULLINE''' | ||
| Line 31: | Line 28: | ||
[[Category: Grutter, M G.]] | [[Category: Grutter, M G.]] | ||
[[Category: Vasak, M.]] | [[Category: Vasak, M.]] | ||
[[Category: | [[Category: Acetylation]] | ||
[[Category: | [[Category: Adma]] | ||
[[Category: | [[Category: Ddah i]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: | [[Category: Metal-binding]] | ||
[[Category: | [[Category: Mma]] | ||
[[Category: | [[Category: No]] | ||
[[Category: | [[Category: No]] | ||
[[Category: | [[Category: S-nitrosylation]] | ||
[[Category: | [[Category: Zinc]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:21:24 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 21:21, 3 May 2008
CRYSTAL STRUCTURE OF DIMETHYLARGININE DIMETHYLAMINOHYDROLASE I IN COMPLEX WITH CITRULLINE
OverviewOverview
Dimethylarginine dimethylaminohydrolase (DDAH) is involved in the regulation of nitric oxide synthase (NOS) by metabolizing the free endogenous arginine derivatives N(omega)-methyl-L-arginine (MMA) and N(omega),N(omega)-dimethyl-L-arginine (ADMA), which are competitive inhibitors of NOS. Here, we present high-resolution crystal structures of DDAH isoform 1 (DDAH-1) isolated from bovine brain in complex with different inhibitors, including S-nitroso-L-homocysteine and Zn2+, a regulator of this mammalian enzyme. The structure of DDAH-1 consists of a propeller-like fold similar to other arginine-modifying enzymes and a flexible loop, which adopts different conformations and acts as a lid at the entrance of the active site. The orientation and interaction mode of inhibitors in the active site give insight into the regulation and the molecular mechanism of the enzyme. The presented structures provide a basis for the structure-based development of specific DDAH-1 inhibitors that might be useful in the therapeutic treatment of NOS dysfunction-related diseases.
About this StructureAbout this Structure
2C6Z is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the mammalian NOS regulator dimethylarginine dimethylaminohydrolase: A basis for the design of specific inhibitors., Frey D, Braun O, Briand C, Vasak M, Grutter MG, Structure. 2006 May;14(5):901-11. PMID:16698551 Page seeded by OCA on Sat May 3 21:21:24 2008