2c39: Difference between revisions

Revision as of 21:10, 3 May 2008

RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP

OverviewOverview

The exosome is a macromolecular complex that plays fundamental roles in the biogenesis and turnover of a large number of RNA species. Here we report the crystal structures of the Rrp41-Rrp42 core complex of the S. solfataricus exosome bound to short single-stranded RNAs and to ADP. The RNA binding cleft recognizes four nucleotides in a sequence-unspecific manner, mainly by electrostatic interactions with the phosphate groups. Interactions at the 2' hydroxyls of the sugars provide specificity for RNA over DNA. The structures show both the bound substrate and the cleaved product of the reaction, suggesting a catalytic mechanism for the 3'-5' phosphorolytic activity of the exosome.

About this StructureAbout this Structure

2C39 is a Protein complex structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:16285928 Page seeded by OCA on Sat May 3 21:10:57 2008

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OCA

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 [[Image:2c39.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2c39 |SIZE=350|CAPTION= <scene name='initialview01'>2c39</scene>, resolution 3.30&Aring;
+The line below this paragraph, containing "STRUCTURE_2c39", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+X'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2c39|  PDB=2c39  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c39 OCA], [http://www.ebi.ac.uk/pdbsum/2c39 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c39 RCSB]</span>
-}}
 '''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP'''
@@ Line 27: / Line 24: @@
 [[Category: Conti, E.]]
 [[Category: Lorentzen, E.]]
-[[Category: archaeal]]
+[[Category: Archaeal]]
-[[Category: exonuclease]]
+[[Category: Exonuclease]]
-[[Category: exoribonuclease]]
+[[Category: Exoribonuclease]]
-[[Category: exosome]]
+[[Category: Exosome]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: nuclease]]
+[[Category: Nuclease]]
-[[Category: phosphorolytic]]
+[[Category: Phosphorolytic]]
-[[Category: rna degradation]]
+[[Category: Rna degradation]]
-[[Category: rnase ph]]
+[[Category: Rnase ph]]
-[[Category: rrp41]]
+[[Category: Rrp41]]
-[[Category: rrp42]]
+[[Category: Rrp42]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:10:57 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:15:40 2008''