2c2u: Difference between revisions

Revision as of 21:09, 3 May 2008

DPS FROM DEINOCOCCUS RADIODURANS

OverviewOverview

The crystal structure of a DNA-binding protein from starved cells (Dps) (DR2263) from Deinococcus radiodurans was determined in two states: a native form, to 1.1-A resolution, and one soaked in an iron solution, to 1.6-A resolution. In comparison with other Dps proteins, DR2263 has an extended N-terminal extension, in both structures presented here, a novel metal binding site was identified in this N-terminal extension and was assigned to bound zinc. The zinc is tetrahedrally coordinated and the ligands, that belong to the N-terminal extension, are two histidines, one glutamate and one aspartate residue, which are unique to this protein within the Dps family. In the iron-soaked crystal structure, a total of three iron sites per monomer were found: one site corresponds to the ferroxidase centre with structural similarities to those found in other Dps family members; the two other sites are located on the two different threefold axes corresponding to small pores in the Dps sphere, which may possibly form the entrance and exit channels for iron storage.

About this StructureAbout this Structure

2C2U is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of Deinococcus radiodurans Dps protein (DR2263) reveals the presence of a novel metal centre in the N terminus., Romao CV, Mitchell EP, McSweeney S, J Biol Inorg Chem. 2006 Oct;11(7):891-902. Epub 2006 Jul 20. PMID:16855817 Page seeded by OCA on Sat May 3 21:09:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2c2u.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2c2u |SIZE=350|CAPTION= <scene name='initialview01'>2c2u</scene>, resolution 1.10&Aring;
+The line below this paragraph, containing "STRUCTURE_2c2u", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Fe+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2c2u|  PDB=2c2u  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c2u OCA], [http://www.ebi.ac.uk/pdbsum/2c2u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c2u RCSB]</span>
-}}
 '''DPS FROM DEINOCOCCUS RADIODURANS'''
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 [[Category: Mitchell, E.]]
 [[Category: Romao, C V.]]
-[[Category: deinococcus radioduran]]
+[[Category: Deinococcus radioduran]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: dp]]
+[[Category: Dp]]
-[[Category: iron]]
+[[Category: Iron]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:09:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:15:28 2008''