2c2l: Difference between revisions
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'''CRYSTAL STRUCTURE OF THE CHIP U-BOX E3 UBIQUITIN LIGASE''' | '''CRYSTAL STRUCTURE OF THE CHIP U-BOX E3 UBIQUITIN LIGASE''' | ||
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[[Category: Roe, S M.]] | [[Category: Roe, S M.]] | ||
[[Category: Zhang, M.]] | [[Category: Zhang, M.]] | ||
[[Category: | [[Category: E3 ligase]] | ||
[[Category: | [[Category: Heat-shock protein complex]] | ||
[[Category: | [[Category: Tpr]] | ||
[[Category: | [[Category: Ubiquitinylation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:09:08 2008'' | |||
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Revision as of 21:09, 3 May 2008
CRYSTAL STRUCTURE OF THE CHIP U-BOX E3 UBIQUITIN LIGASE
OverviewOverview
CHIP is a dimeric U box E3 ubiquitin ligase that binds Hsp90 and/or Hsp70 via its TPR-domain, facilitating ubiquitylation of chaperone bound client proteins. We have determined the crystal structure of CHIP bound to an Hsp90 C-terminal decapeptide. The structure explains how CHIP associates with either chaperone type and reveals an unusual asymmetric homodimer in which the protomers adopt radically different conformations. Additionally, we identified CHIP as a functional partner of Ubc13-Uev1a in formation of Lys63-linked polyubiquitin chains, extending CHIP's roles into ubiquitin regulation as well as targeted destruction. The structure of Ubc13-Uev1a bound to the CHIP U box domain defines the basis for selective cooperation of CHIP with specific ubiquitin-conjugating enzymes. Remarkably, the asymmetric arrangement of the TPR domains in the CHIP dimer occludes one Ubc binding site, so that CHIP operates with half-of-sites activity, providing an elegant means for coupling a dimeric chaperone to a single ubiquitylation system.
About this StructureAbout this Structure
2C2L is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex., Zhang M, Windheim M, Roe SM, Peggie M, Cohen P, Prodromou C, Pearl LH, Mol Cell. 2005 Nov 23;20(4):525-38. PMID:16307917 Page seeded by OCA on Sat May 3 21:09:08 2008