2c1m: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2c1m.gif|left|200px]] | [[Image:2c1m.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2c1m", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
| | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| | or leave the SCENE parameter empty for the default display. | ||
--> | |||
{{STRUCTURE_2c1m| PDB=2c1m | SCENE= }} | |||
}} | |||
'''NUP50:IMPORTIN-ALPHA COMPLEX''' | '''NUP50:IMPORTIN-ALPHA COMPLEX''' | ||
| Line 27: | Line 24: | ||
[[Category: Matsuura, Y.]] | [[Category: Matsuura, Y.]] | ||
[[Category: Stewart, M.]] | [[Category: Stewart, M.]] | ||
[[Category: | [[Category: Importin-alpha]] | ||
[[Category: | [[Category: Nuclear transport/complex nup50,nuclear protein]] | ||
[[Category: | [[Category: Nucleoporin nup50]] | ||
[[Category: | [[Category: Protein transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:06:22 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 21:06, 3 May 2008
NUP50:IMPORTIN-ALPHA COMPLEX
OverviewOverview
Nuclear import of proteins containing classical nuclear localization signals (NLS) is mediated by the importin-alpha:beta complex that binds cargo in the cytoplasm and facilitates its passage through nuclear pores, after which nuclear RanGTP dissociates the import complex and the importins are recycled. In vertebrates, import is stimulated by nucleoporin Nup50, which has been proposed to accompany the import complex through nuclear pores. However, we show here that the Nup50 N-terminal domain actively displaces NLSs from importin-alpha, which would be more consistent with Nup50 functioning to coordinate import complex disassembly and importin recycling. The crystal structure of the importin-alpha:Nup50 complex shows that Nup50 binds at two sites on importin-alpha. One site overlaps the secondary NLS-binding site, whereas the second extends along the importin-alpha C-terminus. Mutagenesis indicates that interaction at both sites is required for Nup50 to displace NLSs. The Cse1p:Kap60p:RanGTP complex structure suggests how Nup50 is then displaced on formation of the importin-alpha export complex. These results provide a rationale for understanding the series of interactions that orchestrate the terminal steps of nuclear protein import.
About this StructureAbout this Structure
2C1M is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling., Matsuura Y, Stewart M, EMBO J. 2005 Nov 2;24(21):3681-9. Epub 2005 Oct 13. PMID:16222336 Page seeded by OCA on Sat May 3 21:06:22 2008