2c0h: Difference between revisions

Revision as of 21:03, 3 May 2008

X-RAY STRUCTURE OF BETA-MANNANASE FROM BLUE MUSSEL MYTILUS EDULIS

OverviewOverview

Endo-beta-1,4-d-mannanase is the key depolymerizing enzyme for beta-1,4-mannan polymers present in the cell walls of plants and some algae, as well as in some types of plant seeds. Endo-1,4-beta-mannanase from blue mussel Mytilus edulis (MeMan5A) belongs to the glycoside hydrolase (GH) family 5 enzymes. The MeMan5A structure has been determined to 1.6A resolution using the multiple-wavelength anomalous dispersion method at the selenium K edge with selenomethionyl MeMan5A expressed in the yeast Pichia pastoris. As expected for GH 5 enzymes, the structure showed a (betaalpha)(8)-barrel fold. An unusually large number of histidine side-chains are exposed on the surface, which may relate to its location within the crystalline style of the digestive tract of the mussel. Kinetic analysis of MeMan5A revealed that the enzyme requires at least six subsites for efficient hydrolysis. Mannotetraose (M4) and mannopentaose (M5) were shown to interact with subsites -3 to +1, and -3 to +2, respectively. A clear kinetic threshold was observed when going from M4 to M5, indicating that the +2 subsite provides important interaction in the hydrolysis of short oligomeric mannose substrates. The catalytic centre motif at subsite -1 found in superfamily GH clan A is, as expected, conserved in MeMan5A, but the architecture of the catalytic cleft differs significantly from other GH 5 enzyme structures. We therefore suggest that MeMan5A represents a new subfamily in GH 5.

About this StructureAbout this Structure

2C0H is a Single protein structure of sequence from Mytilus edulis. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional crystal structure and enzymic characterization of beta-mannanase Man5A from blue mussel Mytilus edulis., Larsson AM, Anderson L, Xu B, Munoz IG, Uson I, Janson JC, Stalbrand H, Stahlberg J, J Mol Biol. 2006 Apr 14;357(5):1500-10. Epub 2006 Jan 31. PMID:16487541 Page seeded by OCA on Sat May 3 21:03:31 2008

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OCA

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 [[Image:2c0h.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] </span>
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 '''X-RAY STRUCTURE OF BETA-MANNANASE FROM BLUE MUSSEL MYTILUS EDULIS'''
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 [[Category: Uson, I.]]
 [[Category: Xu, B.]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: mytilus eduli]]
+[[Category: Mytilus eduli]]
-[[Category: signal]]
+[[Category: Signal]]
-[[Category: tim alpha/beta barrel]]
+[[Category: Tim alpha/beta barrel]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:03:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:14:29 2008''