2bof: Difference between revisions

Revision as of 20:34, 3 May 2008

CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA IN COMPLEX WITH CELLOTETROSE

OverviewOverview

Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution.

About this StructureAbout this Structure

2BOF is a Single protein structure of sequence from Thermobifida fusca. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060 Page seeded by OCA on Sat May 3 20:34:23 2008

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OCA

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 [[Image:2bof.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2bof |SIZE=350|CAPTION= <scene name='initialview01'>2bof</scene>, resolution 1.64&Aring;
+The line below this paragraph, containing "STRUCTURE_2bof", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+For+Chain+X'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bof OCA], [http://www.ebi.ac.uk/pdbsum/2bof PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bof RCSB]</span>
-}}
 '''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA IN COMPLEX WITH CELLOTETROSE'''
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 [[Category: Roos, A.]]
 [[Category: Wilson, D B.]]
-[[Category: endoglucanase]]
+[[Category: Endoglucanase]]
-[[Category: glycoside hydrolase family 6]]
+[[Category: Glycoside hydrolase family 6]]
-[[Category: methyl cellobiosyl-4-thio-beta-cellobioside]]
+[[Category: Methyl cellobiosyl-4-thio-beta-cellobioside]]
-[[Category: thermobifida fusca]]
+[[Category: Thermobifida fusca]]
-[[Category: tim a/b fold]]
+[[Category: Tim a/b fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:34:23 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:09:24 2008''