2bm7: Difference between revisions

Revision as of 20:29, 3 May 2008

THE STRUCTURE OF MFPA (RV3361C, P3221 CRYSTAL FORM). THE PENTAPEPTIDE REPEAT PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS FOLDS AS A RIGHT-HANDED QUADRILATERAL BETA-HELIX.

OverviewOverview

Fluoroquinolones are gaining increasing importance in the treatment of tuberculosis. The expression of MfpA, a member of the pentapeptide repeat family of proteins from Mycobacterium tuberculosis, causes resistance to ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed quadrilateral beta helix, that exhibits size, shape, and electrostatic similarity to B-form DNA. This represents a form of DNA mimicry and explains both its inhibitory effect on DNA gyrase and fluoroquinolone resistance resulting from the protein's expression in vivo.

About this StructureAbout this Structure

2BM7 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA., Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS, Science. 2005 Jun 3;308(5727):1480-3. PMID:15933203 Page seeded by OCA on Sat May 3 20:29:03 2008

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OCA

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 [[Image:2bm7.gif|left|200px]]
- {{Structure
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 '''THE STRUCTURE OF MFPA (RV3361C, P3221 CRYSTAL FORM). THE PENTAPEPTIDE REPEAT PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS FOLDS AS A RIGHT-HANDED QUADRILATERAL BETA-HELIX.'''
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 [[Category: Takiff, H E.]]
 [[Category: Vetting, M W.]]
-[[Category: dna gyrase]]
+[[Category: Dna gyrase]]
-[[Category: dna mimicry]]
+[[Category: Dna mimicry]]
-[[Category: pentapeptide repeat protein,fluroquinolone resistance]]
+[[Category: Pentapeptide repeat protein,fluroquinolone resistance]]
-[[Category: right-handed quadrilateral beta-helix]]
+[[Category: Right-handed quadrilateral beta-helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:29:03 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:08:31 2008''