2bjw: Difference between revisions

Revision as of 20:23, 3 May 2008

PSPF AAA DOMAIN

OverviewOverview

Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

About this StructureAbout this Structure

2BJW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859 Page seeded by OCA on Sat May 3 20:23:27 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2bjw.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2bjw |SIZE=350|CAPTION= <scene name='initialview01'>2bjw</scene>, resolution 1.75&Aring;
+The line below this paragraph, containing "STRUCTURE_2bjw", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2bjw|  PDB=2bjw  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjw OCA], [http://www.ebi.ac.uk/pdbsum/2bjw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bjw RCSB]</span>
-}}
 '''PSPF AAA DOMAIN'''
@@ Line 35: / Line 32: @@
 [[Category: Wigneshweraraj, S.]]
 [[Category: Zhang, X.]]
-[[Category: aaa,transcription activation]]
+[[Category: Aaa,transcription activation]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: enhancer binding protein]]
+[[Category: Enhancer binding protein]]
-[[Category: gene regulation]]
+[[Category: Gene regulation]]
-[[Category: pspf]]
+[[Category: Pspf]]
-[[Category: sigma54 activator]]
+[[Category: Sigma54 activator]]
-[[Category: transcription regulation,]]
+[[Category: Transcription regulation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:23:27 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:29 2008''