2bay: Difference between revisions
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'''Crystal structure of the Prp19 U-box dimer''' | '''Crystal structure of the Prp19 U-box dimer''' | ||
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[[Category: Oldham, M L.]] | [[Category: Oldham, M L.]] | ||
[[Category: Rosenberg, J A.]] | [[Category: Rosenberg, J A.]] | ||
[[Category: | [[Category: E3 ligase]] | ||
[[Category: | [[Category: Prp19]] | ||
[[Category: | [[Category: U-box]] | ||
[[Category: | [[Category: Ubiquitin ligase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:03:22 2008'' | |||
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Revision as of 20:03, 3 May 2008
Crystal structure of the Prp19 U-box dimer
OverviewOverview
Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases. Prp19 forms a tetramer via a central coiled-coil domain. Here, we show the U-box domain of Prp19 exists as a dimer within the context of the Prp19 tetramer. A high-resolution structure of the homodimeric state of the Prp19 U-box was determined by X-ray crystallography. Mutation of the U-box dimer interface abrogates U-box dimer formation and is lethal in vivo. The structure of the U-box dimer enables construction of a complete model of Prp19 providing insights into how the tetrameric protein functions as an E3 ligase. Finally, comparison of the Prp19 U-box homodimer with the heterodimeric complex of BRCA1/BARD1 RING-finger domains uncovers a common architecture for a family of oligomeric U-box and RING-finger E3 ubiquitin ligases, which has mechanistic implications for E3 ligase-mediated polyubiquitination and E4 polyubiquitin ligases.
About this StructureAbout this Structure
2BAY is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases., Vander Kooi CW, Ohi MD, Rosenberg JA, Oldham ML, Newcomer ME, Gould KL, Chazin WJ, Biochemistry. 2006 Jan 10;45(1):121-30. PMID:16388587 Page seeded by OCA on Sat May 3 20:03:22 2008