2b7o: Difference between revisions

Revision as of 19:57, 3 May 2008

The Structure of 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Mycobacterium tuberculosis

OverviewOverview

The shikimate pathway, responsible for the biosynthesis of aromatic compounds, is essential for the growth of Mycobacterium tuberculosis and is a potential target for the design of new anti-tuberculosis drugs. The first step of this pathway is catalyzed by 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAH7PS). The DAH7PSs have been classified into two apparently unrelated types and, whereas structural data have been obtained for the type I DAH7PSs, no structural information is available for their type II counterparts. The type II DAH7PS from M.tuberculosis has been expressed in Escherichia coli, purified, functionally characterized and crystallized. It is found to be metal ion-dependent and subject to feedback inhibition by phenylalanine, tryptophan, tyrosine and chorismate, with a significant synergistic effect when tryptophan is used in combination with phenylalanine. The crystal structure of M.tuberculosis DAH7PS has been determined by single-wavelength anomalous diffraction and refined at 2.3A in complex with substrate phosphoenolpyruvate and Mn(2+). The structure reveals a tightly associated dimer of (beta/alpha)(8) TIM barrels. The monomer fold, the arrangement of key residues in the active site, and the binding modes of PEP and Mn(2+), all match those of the type I enzymes, and indicate a common ancestry for the type I and type II DAH7PSs, despite their minimal sequence identity. In contrast, the structural elements that decorate the core (beta/alpha)(8) fold differ from those in the type I enzymes, consistent with their different regulatory and oligomeric properties.

About this StructureAbout this Structure

2B7O is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

The structure of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase from Mycobacterium tuberculosis reveals a common catalytic scaffold and ancestry for type I and type II enzymes., Webby CJ, Baker HM, Lott JS, Baker EN, Parker EJ, J Mol Biol. 2005 Dec 9;354(4):927-39. Epub 2005 Oct 21. PMID:16288916 Page seeded by OCA on Sat May 3 19:57:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2b7o.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2b7o |SIZE=350|CAPTION= <scene name='initialview01'>2b7o</scene>, resolution 2.30&Aring;
+The line below this paragraph, containing "STRUCTURE_2b7o", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CE1:O-DODECANYL+OCTAETHYLENE+GLYCOL'>CE1</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= Rv2178c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+-->
-|DOMAIN=
+{{STRUCTURE_2b7o|  PDB=2b7o  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b7o OCA], [http://www.ebi.ac.uk/pdbsum/2b7o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b7o RCSB]</span>
-}}
 '''The Structure of 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Mycobacterium tuberculosis'''
@@ Line 31: / Line 28: @@
 [[Category: Webby, C J.]]
 [[Category: XMTB, Mycobacterium Tuberculosis Structural Proteomics Project.]]
-[[Category: aromatic biosynthesis]]
+[[Category: Aromatic biosynthesis]]
-[[Category: dah7ps synthase]]
+[[Category: Dah7ps synthase]]
-[[Category: evolutionary relationship]]
+[[Category: Evolutionary relationship]]
-[[Category: mycobacterium tuberculosis]]
+[[Category: Mycobacterium tuberculosis]]
-[[Category: mycobacterium tuberculosis structural proteomics project]]
+[[Category: Mycobacterium tuberculosis structural proteomics project]]
-[[Category: shikimate pathway]]
+[[Category: Shikimate pathway]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: xmtb]]
+[[Category: Xmtb]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:57:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:02:35 2008''