2b3l: Difference between revisions

Revision as of 19:48, 3 May 2008

Crystal structure of type I human methionine aminopeptidase in the apo form

OverviewOverview

Determination of the crystal structure of human MetAP1 makes it possible, for the first time, to compare the structures of a Type I and a Type II methionine aminopeptidase (MetAP) from the same organism. Comparison of the Type I enzyme with the previously reported complex of ovalicin with Type II MetAP shows that the active site of the former is reduced in size and would incur steric clashes with the bound inhibitor. This explains why ovalicin and related anti-angiogenesis inhibitors target Type II human MetAP but not Type I. The differences in both size and shape of the active sites between MetAP1 and MetAP2 also help to explain their different substrate specificity. In the presence of excess Co(2+), a third cobalt ion binds in the active site region, explaining why metal ions in excess can be inhibitory. Also, the N-terminal region of the protein contains three distinct Pro-x-x-Pro motifs, supporting the prior suggestion that this region of the protein may participate in binding to the ribosome.

About this StructureAbout this Structure

2B3L is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the functional differences between type I and type II human methionine aminopeptidases., Addlagatta A, Hu X, Liu JO, Matthews BW, Biochemistry. 2005 Nov 15;44(45):14741-9. PMID:16274222 Page seeded by OCA on Sat May 3 19:48:38 2008

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OCA

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 [[Image:2b3l.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2b3l |SIZE=350|CAPTION= <scene name='initialview01'>2b3l</scene>, resolution 1.5&Aring;
+The line below this paragraph, containing "STRUCTURE_2b3l", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= METAP1, KIAA0094 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2b3l|  PDB=2b3l  |  SCENE=  }}
-|RELATEDENTRY=[[2b3h|2B3H]], [[2b3k|2B3K]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3l OCA], [http://www.ebi.ac.uk/pdbsum/2b3l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b3l RCSB]</span>
-}}
 '''Crystal structure of type I human methionine aminopeptidase in the apo form'''
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 [[Category: Liu, J O.]]
 [[Category: Matthews, B W.]]
-[[Category: human]]
+[[Category: Human]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: metalloprotease]]
+[[Category: Metalloprotease]]
-[[Category: methionine aminopeptidase]]
+[[Category: Methionine aminopeptidase]]
-[[Category: pitabread fold]]
+[[Category: Pitabread fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:48:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:00:59 2008''