2ayn: Difference between revisions

Revision as of 19:37, 3 May 2008

Structure of USP14, a proteasome-associated deubiquitinating enzyme

OverviewOverview

The ubiquitin-specific processing protease (UBP) family of deubiquitinating enzymes plays an essential role in numerous cellular processes. Mammalian USP14 (Ubp6 in yeast) is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. Here, we report the crystal structures of the 45-kDa catalytic domain of USP14 in isolation and in a complex with ubiquitin aldehyde, which reveal distinct structural features. In the absence of ubiquitin binding, the catalytic cleft leading to the active site of USP14 is blocked by two surface loops. Binding by ubiquitin induces a significant conformational change that translocates the two surface loops thereby allowing access of the ubiquitin C-terminus to the active site. These structural observations, in conjunction with biochemical characterization, identify important regulatory mechanisms for USP14.

About this StructureAbout this Structure

2AYN is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14., Hu M, Li P, Song L, Jeffrey PD, Chenova TA, Wilkinson KD, Cohen RE, Shi Y, EMBO J. 2005 Nov 2;24(21):3747-56. Epub 2005 Oct 6. PMID:16211010 Page seeded by OCA on Sat May 3 19:37:55 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2ayn.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= USP14, TGT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ayn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayn OCA], [http://www.ebi.ac.uk/pdbsum/2ayn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ayn RCSB]</span>
-}}
 '''Structure of USP14, a proteasome-associated deubiquitinating enzyme'''
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 [[Category: Li, P.]]
 [[Category: Shi, Y.]]
-[[Category: deubiquitinating enzyme]]
+[[Category: Deubiquitinating enzyme]]
-[[Category: dub]]
+[[Category: Dub]]
-[[Category: enzyme mechanism]]
+[[Category: Enzyme mechanism]]
-[[Category: proteasome]]
+[[Category: Proteasome]]
-[[Category: usp14]]
+[[Category: Usp14]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:37:55 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:59:05 2008''