2anx: Difference between revisions

Revision as of 19:16, 3 May 2008

crystal structure of bacteriophage P22 lysozyme mutant L87M

OverviewOverview

The X-ray crystal structure of the previously unknown bacteriophage P22 lysozyme, the product of gene 19, has been determined ab initio by direct methods using the program SIR2002. The presence of several partially occupied iodine anions and samarium cations augmented the ability of direct methods to locate all 2268 non-H protein atoms in the asymmetric unit, making this one of the largest structures to date to be determined ab initio. The iodides were introduced from a quick soak, which the crystal survived sufficiently well to diffract to 1.04 angstroms resolution. The complete heavy-atom substructure contributed 6.6% of the total scattering power. The initial determination of the structure assumed that there were two iodide ions in the asymmetric unit, although it was later determined that these sites correspond to partially occupied samarium ions. Tests suggested that it is better to overestimate rather than underestimate the heavy-atom content. While experimental phases from all of the successful tests were of high quality, the best results came from a SAD experiment using the programs SHELXD and SHELXE. Nonetheless, ab initio structure determination by direct methods was found to be a viable alternative to traditional protein crystallographic methods provided that the X-ray data extend to atomic resolution and heavy atoms with sufficient scattering power are present in the crystal.

About this StructureAbout this Structure

2ANX is a Single protein structure of sequence from Enterobacteria phage p22. Full crystallographic information is available from OCA.

ReferenceReference

Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme., Mooers BH, Matthews BW, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):165-76. Epub 2006, Jan 18. PMID:16421448 Page seeded by OCA on Sat May 3 19:16:15 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2anx.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2anx |SIZE=350|CAPTION= <scene name='initialview01'>2anx</scene>, resolution 1.04&Aring;
+The line below this paragraph, containing "STRUCTURE_2anx", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= 19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])
+-->
-|DOMAIN=
+{{STRUCTURE_2anx|  PDB=2anx  |  SCENE=  }}
-|RELATEDENTRY=[[2anv|2ANV]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2anx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2anx OCA], [http://www.ebi.ac.uk/pdbsum/2anx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2anx RCSB]</span>
-}}
 '''crystal structure of bacteriophage P22 lysozyme mutant L87M'''
@@ Line 28: / Line 25: @@
 [[Category: Matthews, B W.]]
 [[Category: Mooers, B H.]]
-[[Category: atomic resolution]]
+[[Category: Atomic resolution]]
-[[Category: phage lysozyme]]
+[[Category: Phage lysozyme]]
-[[Category: sm-sad]]
+[[Category: Sm-sad]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:16:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:55:06 2008''