2afp: Difference between revisions

Revision as of 18:59, 3 May 2008

THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY

OverviewOverview

A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H- inverted question mark15N inverted question mark heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%.

About this StructureAbout this Structure

2AFP is a Single protein structure of sequence from Hemitripterus americanus. Full crystallographic information is available from OCA.

ReferenceReference

The solution structure of type II antifreeze protein reveals a new member of the lectin family., Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sonnichsen FD, Davies PL, Sykes BD, Biochemistry. 1998 Apr 7;37(14):4712-21. PMID:9537986 Page seeded by OCA on Sat May 3 18:59:48 2008

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OCA

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 '''THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY'''
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 [[Category: Sonnichsen, F D.]]
 [[Category: Sykes, B D.]]
-[[Category: c-type lectin]]
+[[Category: C-type lectin]]
-[[Category: recombinant sea raven protein]]
+[[Category: Recombinant sea raven protein]]
-[[Category: solution backbone fold]]
+[[Category: Solution backbone fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:59:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:52:07 2008''