2aa9: Difference between revisions

Revision as of 18:48, 3 May 2008

EPSP synthase liganded with shikimate

OverviewOverview

The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.

About this StructureAbout this Structure

2AA9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate., Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E, FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867 Page seeded by OCA on Sat May 3 18:48:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2aa9.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2aa9", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span>
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-|GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1g6s|1G6S]], [[1g6t|1G6T]], [[1q36|1Q36]], [[1mi4|1MI4]], [[1x8r|1X8R]], [[1x8t|1X8T]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aa9 OCA], [http://www.ebi.ac.uk/pdbsum/2aa9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aa9 RCSB]</span>
-}}
 '''EPSP synthase liganded with shikimate'''
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 [[Category: Priestman, M A.]]
 [[Category: Schonbrunn, E.]]
-[[Category: inside-out alpha/beta barrel]]
+[[Category: Inside-out alpha/beta barrel]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:48:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:49:59 2008''