2a8q: Difference between revisions

Revision as of 18:45, 3 May 2008

2.6 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese in the Presence of 7-methyl-GDP

OverviewOverview

X29, a 25 kDa Nudix hydrolase from Xenopus laevis that cleaves 5' caps from U8 snoRNA, crystallizes as a homodimeric apoenzyme. Manganese binds crystals of apo-X29 to form holo-X29 only in the presence of nucleot(s)ide. Structural changes in X29 on nucleo-t(s)ide-assisted Mn(+2) uptake account for the observed cooperativity of metal binding. Structures of X29 with GTP or m7GpppA show a different mode of ligand binding from that of other cap binding proteins and suggest a possible three- or four-metal Nudix reaction mechanism. The X29 dimer has no known RNA binding motif, but its striking surface dipolarity and unique structural features create a plausible RNA binding channel on the positive face of the protein. Because U8 snoRNP is essential for accumulation of mature 5.8S and 28S rRNA in vertebrate ribosome biogenesis, and cap structures are required for U8 stability in vivo, X29 could profoundly influence this fundamental cellular pathway.

About this StructureAbout this Structure

2A8Q is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of U8 snoRNA decapping nudix hydrolase, X29, and its metal and cap complexes., Scarsdale JN, Peculis BA, Wright HT, Structure. 2006 Feb;14(2):331-43. PMID:16472752 Page seeded by OCA on Sat May 3 18:45:12 2008

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OCA

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 [[Image:2a8q.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8q OCA], [http://www.ebi.ac.uk/pdbsum/2a8q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a8q RCSB]</span>
-}}
 '''2.6 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese in the Presence of 7-methyl-GDP'''
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 [[Category: Scarsdale, J N.]]
 [[Category: Wright, H T.]]
-[[Category: modified nudix hydrolase fold]]
+[[Category: Modified nudix hydrolase fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:45:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:49:23 2008''