2a5g: Difference between revisions

Revision as of 18:37, 3 May 2008

Cholera toxin A1 subunit bound to ARF6(Q67L)

OverviewOverview

The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.

About this StructureAbout this Structure

2A5G is a Protein complex structure of sequences from Homo sapiens and Vibrio cholerae. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:16099990 Page seeded by OCA on Sat May 3 18:37:52 2008

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OCA

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 [[Image:2a5g.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2a5g |SIZE=350|CAPTION= <scene name='initialview01'>2a5g</scene>, resolution 2.66&Aring;
+The line below this paragraph, containing "STRUCTURE_2a5g", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= ARF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ctxA, toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
+-->
-|DOMAIN=
+{{STRUCTURE_2a5g|  PDB=2a5g  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5g OCA], [http://www.ebi.ac.uk/pdbsum/2a5g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a5g RCSB]</span>
-}}
 '''Cholera toxin A1 subunit bound to ARF6(Q67L)'''
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 Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16099990 16099990]
 [[Category: Homo sapiens]]
-[[Category: NAD(+)--diphthamide ADP-ribosyltransferase]]
 [[Category: Protein complex]]
 [[Category: Vibrio cholerae]]
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 [[Category: Jobling, M G.]]
 [[Category: Neal, C J.O.]]
-[[Category: protein transport/transferase]]
+[[Category: Protein transport/transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:37:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:04 2008''