1zxa: Difference between revisions

Revision as of 18:11, 3 May 2008

Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia

OverviewOverview

Coiled-coil motifs play essential roles in protein assembly and molecular recognition, and are therefore the targets of many ongoing structural and functional studies. However, owing to the dynamic nature of many of the smaller coiled-coil domains, crystallization for X-ray studies is very challenging. Determination of elongated structures using standard NMR approaches is inefficient and usually yields low-resolution structures due to accumulation of small errors over long distances. Here we describe a solution NMR approach based on residual dipolar couplings (RDCs) for rapid and accurate structure determination of coiled-coil dimers. Using this approach, we were able to determine the high-resolution structure of the coiled-coil domain of cGMP-dependent protein kinase Ialpha, a protein of previously unknown structure that is critical for physiological relaxation of vascular smooth muscle. This approach can be extended to solve coiled-coil structures with higher order assemblies.

About this StructureAbout this Structure

1ZXA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha., Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ, Protein Sci. 2005 Sep;14(9):2421-8. PMID:16131665 Page seeded by OCA on Sat May 3 18:11:38 2008

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OCA

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 [[Image:1zxa.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1zxa", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PRKG1, PRKGR1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_1zxa|  PDB=1zxa  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zxa OCA], [http://www.ebi.ac.uk/pdbsum/1zxa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zxa RCSB]</span>
-}}
 '''Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia'''
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 [[Category: Zhou, G P.]]
 [[Category: Zweckstetter, M.]]
-[[Category: parallel coiled coil dimer]]
+[[Category: Parallel coiled coil dimer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:11:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:42:51 2008''