1zot: Difference between revisions

Revision as of 17:53, 3 May 2008

crystal structure analysis of the CyaA/C-Cam with PMEAPP

OverviewOverview

CyaA is crucial for colonization by Bordetella pertussis, the etiologic agent of whooping cough. Here we report crystal structures of the adenylyl cyclase domain (ACD) of CyaA with the C-terminal domain of calmodulin. Four discrete regions of CyaA bind calcium-loaded calmodulin with a large buried contact surface. Of those, a tryptophan residue (W242) at an alpha-helix of CyaA makes extensive contacts with the calcium-induced, hydrophobic pocket of calmodulin. Mutagenic analyses show that all four regions of CyaA contribute to calmodulin binding and the calmodulin-induced conformational change of CyaA is crucial for catalytic activation. A crystal structure of CyaA-calmodulin with adefovir diphosphate, the metabolite of an approved antiviral drug, reveals the location of catalytic site of CyaA and how adefovir diphosphate tightly binds CyaA. The ACD of CyaA shares a similar structure and mechanism of activation with anthrax edema factor (EF). However, the interactions of CyaA with calmodulin completely diverge from those of EF. This provides molecular details of how two structurally homologous bacterial toxins evolved divergently to bind calmodulin, an evolutionarily conserved calcium sensor.

About this StructureAbout this Structure

1ZOT is a Protein complex structure of sequences from Bordetella pertussis and Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin., Guo Q, Shen Y, Lee YS, Gibbs CS, Mrksich M, Tang WJ, EMBO J. 2005 Sep 21;24(18):3190-201. Epub 2005 Sep 1. PMID:16138079 Page seeded by OCA on Sat May 3 17:53:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1zot.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1zot |SIZE=350|CAPTION= <scene name='initialview01'>1zot</scene>, resolution 2.20&Aring;
+The line below this paragraph, containing "STRUCTURE_1zot", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EMA:(ADENIN-9-YL-ETHOXYMETHYL)-HYDROXYPHOSPHINYL-DIPHOSPHATE'>EMA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zot OCA], [http://www.ebi.ac.uk/pdbsum/1zot PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zot RCSB]</span>
-}}
 '''crystal structure analysis of the CyaA/C-Cam with PMEAPP'''
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 [[Category: Guo, Q.]]
 [[Category: Tang, W J.]]
-[[Category: adenylyl cyclase toxin]]
+[[Category: Adenylyl cyclase toxin]]
-[[Category: atp]]
+[[Category: Atp]]
-[[Category: calmodulin-binding]]
+[[Category: Calmodulin-binding]]
-[[Category: cyaa]]
+[[Category: Cyaa]]
-[[Category: pmeapp]]
+[[Category: Pmeapp]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:53:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:39:40 2008''