1zo1: Difference between revisions

Revision as of 17:51, 3 May 2008

IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex

OverviewOverview

The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex.

About this StructureAbout this Structure

1ZO1 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:15935757 Page seeded by OCA on Sat May 3 17:51:57 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1zo1.gif|left|200px]]
- {{Structure
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-}}
 '''IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex'''
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 [[Category: Gursky, R.]]
 [[Category: Zavialov, A.]]
-[[Category: cryo-eletron microscopy]]
+[[Category: Cryo-eletron microscopy]]
-[[Category: e. coli]]
+[[Category: E. coli]]
-[[Category: initiation factor]]
+[[Category: Initiation factor]]
-[[Category: initiation of protein synthesis]]
+[[Category: Initiation of protein synthesis]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:51:57 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:39:25 2008''