1zle: Difference between revisions

Revision as of 17:46, 3 May 2008

Crystal structure of a RGD-containing host-selective toxin: Pyrenophora tritici-repentis Ptr ToxA

OverviewOverview

Tan spot of wheat (Triticum aestivum), caused by the fungus Pyrenophora tritici-repentis, has significant agricultural and economic impact. Ptr ToxA (ToxA), the first discovered proteinaceous host-selective toxin, is produced by certain P. tritici-repentis races and is necessary and sufficient to cause cell death in sensitive wheat cultivars. We present here the high-resolution crystal structure of ToxA in two different crystal forms, providing four independent views of the protein. ToxA adopts a single-domain, beta-sandwich fold of novel topology. Mapping of the existing mutation data onto the structure supports the hypothesized importance of an Arg-Gly-Asp (RGD) and surrounding sequence. Its occurrence in a single, solvent-exposed loop in the protein suggests that it is directly involved in recognition events required for ToxA action. Furthermore, the ToxA structure reveals a surprising similarity with the classic mammalian RGD-containing domain, the fibronectin type III (FnIII) domain: the two topologies are related by circular permutation. The similar topologies and the positional conservation of the RGD-containing loop raises the possibility that ToxA is distantly related to mammalian FnIII proteins and that to gain entry it binds to an integrin-like receptor in the plant host.

About this StructureAbout this Structure

1ZLE is a Single protein structure of sequence from Pyrenophora tritici-repentis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Ptr ToxA: an RGD-containing host-selective toxin from Pyrenophora tritici-repentis., Sarma GN, Manning VA, Ciuffetti LM, Karplus PA, Plant Cell. 2005 Nov;17(11):3190-202. Epub 2005 Oct 7. PMID:16214901 Page seeded by OCA on Sat May 3 17:46:06 2008

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OCA

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 '''Crystal structure of a RGD-containing host-selective toxin: Pyrenophora tritici-repentis Ptr ToxA'''
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 [[Category: Manning, V A.]]
 [[Category: Sarma, G N.]]
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+[[Category: Rgd-motif]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:46:06 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:38:17 2008''