1zjr: Difference between revisions

Revision as of 17:42, 3 May 2008

Crystal Structure of A. aeolicus TrmH/SpoU tRNA modifying enzyme

OverviewOverview

Biological RNAs contain a variety of post-transcriptional modifications that facilitate their efficient function in the cellular environment. One of the two most common forms of modification is methylation of the 2'-hydroxyl group of the ribose sugar, which is performed by a number of S-adenosylmethionine (SAM) dependent methyltransferases. In bacteria, many of these modifications in tRNA and rRNA are carried out by the alpha/beta-knot superfamily of enzymes, whose SAM-binding pocket is created by a characteristic deep trefoil knot. TrmH, an enzyme found throughout all three kingdoms of life, modifies the universally conserved guanosine 18 position of tRNA. The crystal structure of TrmH from the thermophilic bacterium Aquifex aeolicus has been determined at 1.85 A resolution using data collected from a synchrotron-radiation source. The protein reveals a fold typical of members of the SpoU clan of proteins, a subfamily of the alpha/beta-knot superfamily, with alpha-helical extensions at the N- and C-termini that are likely to be involved in tRNA binding.

About this StructureAbout this Structure

1ZJR is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus., Pleshe E, Truesdell J, Batey RT, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Aug 1;61(Pt, 8):722-8. Epub 2005 Jul 30. PMID:16511140 Page seeded by OCA on Sat May 3 17:42:50 2008

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OCA

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 [[Image:1zjr.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_guanosine-2'-O-methyltransferase tRNA guanosine-2'-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.34 2.1.1.34] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= trmH, spoU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])
+-->
-|DOMAIN=
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zjr OCA], [http://www.ebi.ac.uk/pdbsum/1zjr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zjr RCSB]</span>
-}}
 '''Crystal Structure of A. aeolicus TrmH/SpoU tRNA modifying enzyme'''
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 [[Category: Aquifex aeolicus]]
 [[Category: Single protein]]
-[[Category: tRNA guanosine-2'-O-methyltransferase]]
+[[Category: TRNA guanosine-2'-O-methyltransferase]]
 [[Category: Batey, R T.]]
 [[Category: Pleshe, E.]]
 [[Category: Truesdell, J.]]
-[[Category: methylase]]
+[[Category: Methylase]]
-[[Category: rna modifying enzyme]]
+[[Category: Rna modifying enzyme]]
-[[Category: topological knot]]
+[[Category: Topological knot]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:42:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:40 2008''