1zij: Difference between revisions

Revision as of 17:40, 3 May 2008

GCN4-LEUCINE ZIPPER CORE MUTANT ASN16ABA IN THE TRIMERIC STATE

OverviewOverview

Each protein sequence generally adopts a single native fold, but the sequence features that confer structural uniqueness are not well understood. To define the basis for structural heterogeneity, we determined the high resolution X-ray crystal structures of a single GCN4 leucine-zipper mutant (Asn 16 to aminobutyric acid) in both dimeric and trimeric coiled-coil conformations. The mutant sequence is accommodated in two distinct structures by forming similarly-shaped packing surfaces with different sets of atoms. The trimer structure, in comparison to a previously-characterized trimeric mutant with substitutions in eight core residues, shows that the twist of individual helices and the helix-helix crossing angles can vary significantly to produce the most favoured packing arrangement.

About this StructureAbout this Structure

1ZIJ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of a single coiled-coil peptide in two oligomeric states reveal the basis for structural polymorphism., Gonzalez L Jr, Brown RA, Richardson D, Alber T, Nat Struct Biol. 1996 Dec;3(12):1002-9. PMID:8946853 Page seeded by OCA on Sat May 3 17:40:08 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1zij.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1zij |SIZE=350|CAPTION= <scene name='initialview01'>1zij</scene>, resolution 2.00&Aring;
+The line below this paragraph, containing "STRUCTURE_1zij", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1zij|  PDB=1zij  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zij OCA], [http://www.ebi.ac.uk/pdbsum/1zij PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zij RCSB]</span>
-}}
 '''GCN4-LEUCINE ZIPPER CORE MUTANT ASN16ABA IN THE TRIMERIC STATE'''
@@ Line 29: / Line 26: @@
 [[Category: Junior, L Gonzalez.]]
 [[Category: Richardson, D.]]
-[[Category: activator]]
+[[Category: Activator]]
-[[Category: amino-acid biosynthesis]]
+[[Category: Amino-acid biosynthesis]]
-[[Category: coiled coil]]
+[[Category: Coiled coil]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: leucine zipper]]
+[[Category: Leucine zipper]]
-[[Category: nuclear protein]]
+[[Category: Nuclear protein]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:40:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:05 2008''