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'''Crystal Structure of Human N-acetylgalactosaminyltransferase (GTA) Complexed with Lactose''' | '''Crystal Structure of Human N-acetylgalactosaminyltransferase (GTA) Complexed with Lactose''' | ||
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[[Category: Rose, N L.]] | [[Category: Rose, N L.]] | ||
[[Category: Seto, N O.]] | [[Category: Seto, N O.]] | ||
[[Category: | [[Category: Blood group]] | ||
[[Category: Gta]] | |||
[[Category: | [[Category: H-antigen]] | ||
[[Category: | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:39:01 2008'' | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 17:39, 3 May 2008
Crystal Structure of Human N-acetylgalactosaminyltransferase (GTA) Complexed with Lactose
OverviewOverview
The human ABO(H) blood group A and B antigens are generated by the homologous glycosyltransferases A (GTA) and B (GTB), which add the monosaccharides GalNAc and Gal, respectively, to the cell-surface H antigens. In the first comprehensive structural study of the recognition by a glycosyltransferase of a panel of substrates corresponding to acceptor fragments, 14 high resolution crystal structures of GTA and GTB have been determined in the presence of oligosaccharides corresponding to different segments of the type I (alpha-l-Fucp-(1-->2)-beta-D-Galp-(1-->3)-beta-D-GlcNAcp-OR, where R is a glycoprotein or glycolipid in natural acceptors) and type II (alpha-l-Fucp-(1-->2)-beta-D-Galp-(1-->4)-beta-d-GlcNAcp-OR) H antigen trisaccharides. GTA and GTB differ in only four "critical" amino acid residues (Arg/Gly-176, Gly/Ser-235, Leu/Met-266, and Gly/Ala-268). As these enzymes both utilize the H antigen acceptors, the four critical residues had been thought to be involved strictly in donor recognition; however, we now report that acceptor binding and subsequent transfer are significantly influenced by two of these residues: Gly/Ser-235 and Leu/Met-266. Furthermore, these structures show that acceptor recognition is dominated by the central Gal residue despite the fact that the L-Fuc residue is required for efficient catalysis and give direct insight into the design of model inhibitors for GTA and GTB.
DiseaseDisease
Known disease associated with this structure: Blood group, ABO system OMIM:[110300]
About this StructureAbout this Structure
1ZI1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Differential recognition of the type I and II H antigen acceptors by the human ABO(H) blood group A and B glycosyltransferases., Letts JA, Rose NL, Fang YR, Barry CH, Borisova SN, Seto NO, Palcic MM, Evans SV, J Biol Chem. 2006 Feb 10;281(6):3625-32. Epub 2005 Dec 2. PMID:16326711 Page seeded by OCA on Sat May 3 17:39:01 2008