1zh6: Difference between revisions

Revision as of 17:37, 3 May 2008

Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine

OverviewOverview

It has been recently shown that orthogonal tRNA/aminoacyl-tRNA synthetase pairs can be evolved to allow genetic incorporation of unnatural amino acids into proteins in both prokaryotes and eukaryotes. Here we describe the crystal structure of an evolved aminoacyl-tRNA synthetase that charges the unnatural amino acid p-acetylphenylalanine. Molecular recognition is due to altered hydrogen bonding and packing interactions with bound substrate that result from changes in both side-chain and backbone conformation.

About this StructureAbout this Structure

1ZH6 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

ReferenceReference

Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase., Turner JM, Graziano J, Spraggon G, Schultz PG, J Am Chem Soc. 2005 Nov 2;127(43):14976-7. PMID:16248607 Page seeded by OCA on Sat May 3 17:37:07 2008

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OCA

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 [[Image:1zh6.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] </span>
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-|GENE= tyrS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1zh0|1ZH0]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zh6 OCA], [http://www.ebi.ac.uk/pdbsum/1zh6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zh6 RCSB]</span>
-}}
 '''Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine'''
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 [[Category: Spraggon, G.]]
 [[Category: Turner, J M.]]
-[[Category: ketone]]
+[[Category: Ketone]]
-[[Category: structural plasticity]]
+[[Category: Structural plasticity]]
-[[Category: trna synthetase]]
+[[Category: Trna synthetase]]
-[[Category: unnatural amino acid]]
+[[Category: Unnatural amino acid]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:37:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:36:29 2008''