1ze1: Difference between revisions

Revision as of 17:30, 3 May 2008

Conformational Change of Pseudouridine 55 Synthase upon Its Association with RNA Substrate

OverviewOverview

Pseudouridine 55 synthase (Psi55S) catalyzes isomerization of uridine (U) to pseudouridine (Psi) at position 55 in transfer RNA. The crystal structures of Thermotoga maritima Psi55S, and its complex with RNA, have been determined at 2.9 and 3.0 A resolutions, respectively. Structural comparisons with other families of pseudouridine synthases (PsiS) indicate that Psi55S may acquire its ability to recognize a stem-loop RNA substrate by two insertions of polypeptides into the PsiS core. The structure of apo-Psi55S reveals that these two insertions interact with each other. However, association with RNA substrate induces substantial conformational change in one of the insertions, resulting in disruption of interaction between insertions and association of both insertions with the RNA substrate. Specific interactions between two insertions, as well as between the insertions and the RNA substrate, account for the molecular basis of the conformational change.

About this StructureAbout this Structure

1ZE1 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

ReferenceReference

Conformational change of pseudouridine 55 synthase upon its association with RNA substrate., Phannachet K, Huang RH, Nucleic Acids Res. 2004 Feb 27;32(4):1422-9. Print 2004. PMID:14990747 Page seeded by OCA on Sat May 3 17:30:54 2008

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OCA

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 '''Conformational Change of Pseudouridine 55 Synthase upon Its Association with RNA Substrate'''
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 [[Category: Huang, R H.]]
 [[Category: Phannachet, K.]]
-[[Category: rna modification enzyme]]
+[[Category: Rna modification enzyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:30:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:34:39 2008''