1zav: Difference between revisions

Revision as of 17:24, 3 May 2008

Ribosomal Protein L10-L12(NTD) Complex, Space Group P21

OverviewOverview

The L7/12 stalk of the large subunit of bacterial ribosomes encompasses protein L10 and multiple copies of L7/12. We present crystal structures of Thermotoga maritima L10 in complex with three L7/12 N-terminal-domain dimers, refine the structure of an archaeal L10E N-terminal domain on the 50S subunit, and identify these elements in cryo-electron-microscopic reconstructions of Escherichia coli ribosomes. The mobile C-terminal helix alpha8 of L10 carries three L7/12 dimers in T. maritima and two in E. coli, in concordance with the different length of helix alpha8 of L10 in these organisms. The stalk is organized into three elements (stalk base, L10 helix alpha8-L7/12 N-terminal-domain complex, and L7/12 C-terminal domains) linked by flexible connections. Highly mobile L7/12 C-terminal domains promote recruitment of translation factors to the ribosome and stimulate GTP hydrolysis by the ribosome bound factors through stabilization of their active GTPase conformation.

About this StructureAbout this Structure

1ZAV is a Protein complex structure of sequences from Thermotoga maritima. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation., Diaconu M, Kothe U, Schlunzen F, Fischer N, Harms JM, Tonevitsky AG, Stark H, Rodnina MV, Wahl MC, Cell. 2005 Jul 1;121(7):991-1004. PMID:15989950 Page seeded by OCA on Sat May 3 17:24:02 2008

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OCA

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 [[Image:1zav.gif|left|200px]]
- {{Structure
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-|GENE= rplJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima]), rplL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
+-->
-|DOMAIN=
+{{STRUCTURE_1zav|  PDB=1zav  |  SCENE=  }}
-|RELATEDENTRY=[[1zaw|1ZAW]], [[1zax|1ZAX]], [[1zb4|1ZB4]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zav OCA], [http://www.ebi.ac.uk/pdbsum/1zav PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zav RCSB]</span>
-}}
 '''Ribosomal Protein L10-L12(NTD) Complex, Space Group P21'''
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 [[Category: Tonevitski, A G.]]
 [[Category: Wahl, M C.]]
-[[Category: cryo-electron microscopy]]
+[[Category: Cryo-electron microscopy]]
-[[Category: gtpase stimulation]]
+[[Category: Gtpase stimulation]]
-[[Category: l10-l12 complex structure]]
+[[Category: L10-l12 complex structure]]
-[[Category: l10e structure]]
+[[Category: L10e structure]]
-[[Category: l7/12 ribosomal stalk]]
+[[Category: L7/12 ribosomal stalk]]
-[[Category: mechanism of translation]]
+[[Category: Mechanism of translation]]
-[[Category: rapid kinetic]]
+[[Category: Rapid kinetic]]
-[[Category: ribosome structure and function]]
+[[Category: Ribosome structure and function]]
-[[Category: thiostrepton loop of 23s rrna]]
+[[Category: Thiostrepton loop of 23s rrna]]
-[[Category: translation factor recruitment]]
+[[Category: Translation factor recruitment]]
-[[Category: x-ray crystallography]]
+[[Category: X-ray crystallography]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:24:02 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:33:24 2008''