1z63: Difference between revisions

Revision as of 17:13, 3 May 2008

Sulfolobus solfataricus SWI2/SNF2 ATPase core in complex with dsDNA

OverviewOverview

SWI2/SNF2 ATPases remodel chromatin or other DNA:protein complexes by a poorly understood mechanism that involves ATP-dependent DNA translocation and generation of superhelical torsion. Crystal structures of a dsDNA-translocating SWI2/SNF2 ATPase core from Sulfolobus solfataricus reveal two helical SWI2/SNF2 specific subdomains, fused to a DExx box helicase-related ATPase core. Fully base paired duplex DNA binds along a central cleft via both minor groove strands, indicating that SWI2/SNF2 ATPases travel along the dsDNA minor groove without strand separation. A structural switch, linking DNA binding and the active site DExx motif, may account for the stimulation of ATPase activity by dsDNA. Our results suggest that torque in remodeling processes is generated by an ATP-driven screw motion of DNA along the active site cleft. The structures also redefine SWI2/SNF2 functional motifs and uncover unexpected structural correlation of mutations in Cockayne and X-linked mental retardation syndromes.

About this StructureAbout this Structure

1Z63 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

ReferenceReference

X-ray structures of the Sulfolobus solfataricus SWI2/SNF2 ATPase core and its complex with DNA., Durr H, Korner C, Muller M, Hickmann V, Hopfner KP, Cell. 2005 May 6;121(3):363-73. PMID:15882619 Page seeded by OCA on Sat May 3 17:13:08 2008

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OCA

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 [[Image:1z63.gif|left|200px]]
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z63 OCA], [http://www.ebi.ac.uk/pdbsum/1z63 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z63 RCSB]</span>
-}}
 '''Sulfolobus solfataricus SWI2/SNF2 ATPase core in complex with dsDNA'''
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 [[Category: Koerner, C.]]
 [[Category: Mueller, M.]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:13:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:29:37 2008''