1z08: Difference between revisions

Revision as of 17:01, 3 May 2008

GppNHp-Bound Rab21 Q53G mutant GTPase

OverviewOverview

Rab GTPases regulate all stages of membrane trafficking, including vesicle budding, cargo sorting, transport, tethering and fusion. In the inactive (GDP-bound) conformation, accessory factors facilitate the targeting of Rab GTPases to intracellular compartments. After nucleotide exchange to the active (GTP-bound) conformation, Rab GTPases interact with functionally diverse effectors including lipid kinases, motor proteins and tethering complexes. How effectors distinguish between homologous Rab GTPases represents an unresolved problem with respect to the specificity of vesicular trafficking. Using a structural proteomic approach, we have determined the specificity and structural basis underlying the interaction of the multivalent effector rabenosyn-5 with the Rab family. The results demonstrate that even the structurally similar effector domains in rabenosyn-5 can achieve highly selective recognition of distinct subsets of Rab GTPases exclusively through interactions with the switch and interswitch regions. The observed specificity is determined at a family-wide level by structural diversity in the active conformation, which governs the spatial disposition of critical conserved recognition determinants, and by a small number of both positive and negative sequence determinants that allow further discrimination between Rab GTPases with similar switch conformations.

About this StructureAbout this Structure

1Z08 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of family-wide Rab GTPase recognition by rabenosyn-5., Eathiraj S, Pan X, Ritacco C, Lambright DG, Nature. 2005 Jul 21;436(7049):415-9. PMID:16034420 Page seeded by OCA on Sat May 3 17:01:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1z08.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1z08 |SIZE=350|CAPTION= <scene name='initialview01'>1z08</scene>, resolution 1.80&Aring;
+The line below this paragraph, containing "STRUCTURE_1z08", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= RAB21, KIAA0118 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_1z08|  PDB=1z08  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z08 OCA], [http://www.ebi.ac.uk/pdbsum/1z08 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z08 RCSB]</span>
-}}
 '''GppNHp-Bound Rab21 Q53G mutant GTPase'''
@@ Line 29: / Line 26: @@
 [[Category: Pan, X.]]
 [[Category: Ritacco, C.]]
-[[Category: protein transport]]
+[[Category: Protein transport]]
-[[Category: rab gtpase]]
+[[Category: Rab gtpase]]
-[[Category: rab21]]
+[[Category: Rab21]]
-[[Category: vesicular trafficking]]
+[[Category: Vesicular trafficking]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:01:36 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:27:08 2008''